Nandrolone reduces activation of Notch signaling in denervated muscle associated with increased Numb expression

Xin Hua Liu, Shen Yao, Rui Fang Qiao, Alice C. Levine, Alexander Kirschenbaum, Jiangping Pan, Yong Wu, Weiping Qin, William A. Bauman, Christopher P. Cardozo

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Nandrolone, an anabolic steroid, slows denervation-atrophy in rat muscle. The molecular mechanisms responsible for this effect are not well understood. Androgens and anabolic steroids activate Notch signaling in animal models of aging and thereby mitigate sarcopenia. To explore the molecular mechanisms by which nandrolone prevents denervation-atrophy, we investigated the effects of nandrolone on Notch signaling in denervated rat gastrocnemius muscle. Denervation significantly increased Notch activity reflected by elevated levels of nuclear Notch intracellular domain (NICD) and expression of Hey1 (a Notch target gene). Activation was greatest at 7 and 35. days after denervation but remained present at 56. days after denervation. Activation of Notch in denervated muscle was prevented by nandrolone associated with upregulated expression of Numb mRNA and protein. These data demonstrate that denervation activates Notch signaling, and that nandrolone abrogates this response associated with increased expression of Numb, suggesting a potential mechanism by which nandrolone reduces denervation-atrophy.

Original languageEnglish
Pages (from-to)165-169
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - 14 Oct 2011


  • Anabolic steroids
  • Androgens
  • Muscle atrophy
  • Nandrolone
  • Notch
  • Numb


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