TY - JOUR
T1 - N-Terminal amino acid sequence of two novel tumor-derived adenylate cyclase-stimulating proteins
T2 - Identification of parathyroid hormone-like and parathyroid hormone-unlike domains
AU - Stewart, Andrew F.
AU - Wu, Terence
AU - Goumas, Douglas
AU - Burtis, William J.
AU - Broadus, Arthur E.
N1 - Funding Information:
The authors wish to thank Ms. Katherine Stone and Drs. Kenneth Williams and Williams Konigsberg in the Protein Sequencing Facility at Yale University School of Medicine for their contribution to this work. We also thank Mrs. Ann Blood for expert manuscript preparation. This work was supported by the Veterans Administration and by NIH grant 30102.
PY - 1987/7/31
Y1 - 1987/7/31
N2 - A 17,000 dalton human adenylate cyclase-stimulating protein has previously been purified from a human tumor associated with humoral hypercalcemia of malignancy. This report describes the purification of a related 7,000-9,000 MW protein from a second tumor, and provides N-terminal amino acid sequence of these two peptides. The sequences of the peptides are identical, defining the smaller peptide as an N-terminal portion of the larger peptide. The two peptides possess one region of strong homology with parathyroid hormone and a second divergent region. These structural similarities and differences may explain the similarities and differences which occur in patients with hyperparathyroidism and humoral hypercalcemia of malignancy.
AB - A 17,000 dalton human adenylate cyclase-stimulating protein has previously been purified from a human tumor associated with humoral hypercalcemia of malignancy. This report describes the purification of a related 7,000-9,000 MW protein from a second tumor, and provides N-terminal amino acid sequence of these two peptides. The sequences of the peptides are identical, defining the smaller peptide as an N-terminal portion of the larger peptide. The two peptides possess one region of strong homology with parathyroid hormone and a second divergent region. These structural similarities and differences may explain the similarities and differences which occur in patients with hyperparathyroidism and humoral hypercalcemia of malignancy.
UR - http://www.scopus.com/inward/record.url?scp=0023669186&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(87)90581-X
DO - 10.1016/0006-291X(87)90581-X
M3 - Article
C2 - 3619898
AN - SCOPUS:0023669186
SN - 0006-291X
VL - 146
SP - 672
EP - 678
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -