@inbook{261fe5af60184f4ea6492d5143f1435d,
title = "Multiplex Immunostaining to Spatially Resolve the Cellular Landscape in Human and Mouse Livers",
abstract = "Histological techniques based on tissue colorations (e.g., hematoxylin-eosin, Sirius red) and immunostaining remain gold standard methodologies for diagnostic or phenotyping purposes in liver disease research and clinical hepatology. With the development of -omics technologies, greater information can be extracted from tissue sections. We describe a sequential immunostaining protocol consisting of repetitive cycles of immunostaining and chemically induced antibody stripping that can be readily applied to various formalin-fixed tissues (liver or other organs, mouse or human) and does not require specific equipment or commercial kits. Importantly, the combination of antibodies can be adapted according to specific clinical or scientific needs.",
keywords = "Antibodies, Antibody stripping, Imaging cytometry, Immune microenvironment, Liver fibrosis, Multiplex immunostaining, Tissue section",
author = "Adrien Guillot and Kohlhepp, \{Marlene Sophia\} and Frank Tacke",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-3207-9\_15",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "245--255",
booktitle = "Methods in Molecular Biology",
}