Multifactorial seroprofiling dissects the contribution of pre-existing human coronaviruses responses to SARS-CoV-2 immunity

Irene A. Abela, Chloé Pasin, Magdalena Schwarzmüller, Selina Epp, Michèle E. Sickmann, Merle M. Schanz, Peter Rusert, Jacqueline Weber, Stefan Schmutz, Annette Audigé, Liridona Maliqi, Annika Hunziker, Maria C. Hesselman, Cyrille R. Niklaus, Jochen Gottschalk, Eméry Schindler, Alexander Wepf, Urs Karrer, Aline Wolfensberger, Silvana K. RampiniPatrick M. Meyer Sauteur, Christoph Berger, Michael Huber, Jürg Böni, Dominique L. Braun, Maddalena Marconato, Markus G. Manz, Beat M. Frey, Huldrych F. Günthard, Roger D. Kouyos, Alexandra Trkola

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Determination of SARS-CoV-2 antibody responses in the context of pre-existing immunity to circulating human coronavirus (HCoV) is critical for understanding protective immunity. Here we perform a multifactorial analysis of SARS-CoV-2 and HCoV antibody responses in pre-pandemic (N = 825) and SARS-CoV-2-infected donors (N = 389) using a custom-designed multiplex ABCORA assay. ABCORA seroprofiling, when combined with computational modeling, enables accurate definition of SARS-CoV-2 seroconversion and prediction of neutralization activity, and reveals intriguing interrelations with HCoV immunity. Specifically, higher HCoV antibody levels in SARS-CoV-2-negative donors suggest that pre-existing HCoV immunity may provide protection against SARS-CoV-2 acquisition. In those infected, higher HCoV activity is associated with elevated SARS-CoV-2 responses, indicating cross-stimulation. Most importantly, HCoV immunity may impact disease severity, as patients with high HCoV reactivity are less likely to require hospitalization. Collectively, our results suggest that HCoV immunity may promote rapid development of SARS-CoV-2-specific immunity, thereby underscoring the importance of exploring cross-protective responses for comprehensive coronavirus prevention.

Original languageEnglish
Article number6703
JournalNature Communications
Volume12
Issue number1
DOIs
StatePublished - Dec 2021
Externally publishedYes

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