Multibatch cytometry data integration for optimal immunophenotyping

Masato Ogishi, Rui Yang, Conor Gruber, Peng Zhang, Simon J. Pelham, András N. Spaan, Jérémie Rosain, Marwa Chbihi, Ji Eun Han, V. Koneti Rao, Leena Kainulainen, Jacinta Bustamante, Bertrand Boisson, Dusan Bogunovic, Stéphanie Boisson-Dupuis, Jean Laurent Casanova

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

High-dimensional cytometry is a powerful technique for deciphering the immunopathological factors common to multiple individuals. However, rational comparisons of multiple batches of experiments performed on different occasions or at different sites are challenging because of batch effects. In this study, we describe the integration of multibatch cytometry datasets (iMUBAC), a flexible, scalable, and robust computational framework for unsupervised cell-type identification across multiple batches of high-dimensional cytometry datasets, even without technical replicates. After overlaying cells from multiple healthy controls across batches, iMUBAC learns batch-specific cell-type classification boundaries and identifies aberrant immunophenotypes in patient samples from multiple batches in a unified manner. We illustrate unbiased and streamlined immunophenotyping using both public and in-house mass cytometry and spectral flow cytometry datasets. The method is available as the R package iMUBAC (https://github.com/casanova-lab/iMUBAC).

Original languageEnglish
Pages (from-to)206-213
Number of pages8
JournalJournal of Immunology
Volume206
Issue number1
DOIs
StatePublished - 1 Jan 2021

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