@article{ce46f664d36345c69dab295ddb112ad6,
title = "Multi-omics profiling of single nuclei from frozen archived postmortem human pituitary tissue",
abstract = "Concomitant profiling of transcriptome and chromatin accessibility in isolated nuclei can reveal gene regulatory control mechanisms in health and disease. We report a single nucleus multi-omics analysis protocol optimized for frozen archived postmortem human pituitaries that is also effective for frozen ovine and murine pituitaries and human skeletal muscle biopsies. Its main advantages are that (1) it is not limited to fresh tissue, (2) it avoids tissue dissociation-induced transcriptional changes, and (3) it includes a novel, automated quality control pipeline. For complete details on the use and execution of this protocol, please refer to Ruf-Zamojski et al. (2021) and Zhang et al. (2022).",
keywords = "Cell Biology, Genomics, Health Sciences, RNAseq, Sequencing, Single Cell",
author = "Natalia Mendelev and Michel Zamojski and Amper, {Mary Anne S.} and Cheng, {Wan Sze} and Hanna Pincas and Nair, {Venugopalan D.} and Elena Zaslavsky and Sealfon, {Stuart C.} and Frederique Ruf-Zamojski",
note = "Funding Information: This work was supported by funding from the National Institute of Health (NIH) Grant DK46943 (S.C.S.). We acknowledge the New York Genome Center for sequencing. Human tissue was obtained from the NIH NeuroBioBank. This work was supported in part through the computational and data resources and staff expertise provided by Scientific Computing at the Icahn School of Medicine at Mount Sinai. Funding Information: This work was supported by funding from the National Institute of Health (NIH) Grant DK46943 (S.C.S.). We acknowledge the New York Genome Center for sequencing. Human tissue was obtained from the NIH NeuroBioBank. This work was supported in part through the computational and data resources and staff expertise provided by Scientific Computing at the Icahn School of Medicine at Mount Sinai. N.M. performed research. H.P. drafted the manuscript. M.Z. W.S.C. and E.Z. performed the QC analysis. M.A.S.A. and V.D.N. sequenced the samples. S.C.S. designed the study, analyzed and interpreted data, and drafted the manuscript. F.R.Z. designed the study, performed the research, analyzed and interpreted data, and drafted the manuscript. All authors edited the manuscript and approved its final version. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2022 The Authors",
year = "2022",
month = jun,
day = "17",
doi = "10.1016/j.xpro.2022.101446",
language = "English",
volume = "3",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",
}