Abstract
Concomitant profiling of transcriptome and chromatin accessibility in isolated nuclei can reveal gene regulatory control mechanisms in health and disease. We report a single nucleus multi-omics analysis protocol optimized for frozen archived postmortem human pituitaries that is also effective for frozen ovine and murine pituitaries and human skeletal muscle biopsies. Its main advantages are that (1) it is not limited to fresh tissue, (2) it avoids tissue dissociation-induced transcriptional changes, and (3) it includes a novel, automated quality control pipeline. For complete details on the use and execution of this protocol, please refer to Ruf-Zamojski et al. (2021) and Zhang et al. (2022).
Original language | English |
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Article number | 101446 |
Journal | STAR Protocols |
Volume | 3 |
Issue number | 2 |
DOIs | |
State | Published - 17 Jun 2022 |
Keywords
- Cell Biology
- Genomics
- Health Sciences
- RNAseq
- Sequencing
- Single Cell