Multi-omics profiling of single nuclei from frozen archived postmortem human pituitary tissue

Natalia Mendelev, Michel Zamojski, Mary Anne S. Amper, Wan Sze Cheng, Hanna Pincas, Venugopalan D. Nair, Elena Zaslavsky, Stuart C. Sealfon, Frederique Ruf-Zamojski

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Concomitant profiling of transcriptome and chromatin accessibility in isolated nuclei can reveal gene regulatory control mechanisms in health and disease. We report a single nucleus multi-omics analysis protocol optimized for frozen archived postmortem human pituitaries that is also effective for frozen ovine and murine pituitaries and human skeletal muscle biopsies. Its main advantages are that (1) it is not limited to fresh tissue, (2) it avoids tissue dissociation-induced transcriptional changes, and (3) it includes a novel, automated quality control pipeline. For complete details on the use and execution of this protocol, please refer to Ruf-Zamojski et al. (2021) and Zhang et al. (2022).

Original languageEnglish
Article number101446
JournalSTAR Protocols
Volume3
Issue number2
DOIs
StatePublished - 17 Jun 2022

Keywords

  • Cell Biology
  • Genomics
  • Health Sciences
  • RNAseq
  • Sequencing
  • Single Cell

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