TY - JOUR
T1 - Mouse cytolytic T lymphocytes induced by xenogeneic rat stimulator cells exhibit specificity for H-2 complex alloantigens
AU - Burakoff, S. J.
AU - Ratnofsky, S. E.
AU - Benacerraf, B.
PY - 1977
Y1 - 1977
N2 - When mouse spleen cells were stimulated with irradiated xenogenic, allogeneic, or trinitrophenyl-modified syngeneic lymphoid cells, the strongest cytolytic response was induced by alloantigens. Mouse cytolytic T lymphocytes generated to rat lymphoid cells demonstrated specificity for the immunizing rat strain, but extensive lysis of allogeneic target cells from certain mouse strains was also observed. Cold target inhibition studies indicated that separate clones of xenoantigen-induced cytolytic T lymphocytes lysed each of the allogeneic murine targets. [3H]Thymidine suicide of the effector cells generated to the rat stimulators revealed that only some of all potentially mouse cytolytic T lymphocyte precursors with specificity for a given allogeneic target are activated by the stimulation with rat cells. This evidence that xenoantigens induce alloreactive cytolytic T lymphocytes is discussed in light of our proposal that the cytolytic T lymphocyte receptor repertoire is directed at variants autologous major histocompatibility complex antigens.
AB - When mouse spleen cells were stimulated with irradiated xenogenic, allogeneic, or trinitrophenyl-modified syngeneic lymphoid cells, the strongest cytolytic response was induced by alloantigens. Mouse cytolytic T lymphocytes generated to rat lymphoid cells demonstrated specificity for the immunizing rat strain, but extensive lysis of allogeneic target cells from certain mouse strains was also observed. Cold target inhibition studies indicated that separate clones of xenoantigen-induced cytolytic T lymphocytes lysed each of the allogeneic murine targets. [3H]Thymidine suicide of the effector cells generated to the rat stimulators revealed that only some of all potentially mouse cytolytic T lymphocyte precursors with specificity for a given allogeneic target are activated by the stimulation with rat cells. This evidence that xenoantigens induce alloreactive cytolytic T lymphocytes is discussed in light of our proposal that the cytolytic T lymphocyte receptor repertoire is directed at variants autologous major histocompatibility complex antigens.
UR - http://www.scopus.com/inward/record.url?scp=0017712712&partnerID=8YFLogxK
U2 - 10.1073/pnas.74.10.4572
DO - 10.1073/pnas.74.10.4572
M3 - Article
C2 - 73180
AN - SCOPUS:0017712712
SN - 0027-8424
VL - 74
SP - 4572
EP - 4576
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 10
ER -