TY - JOUR
T1 - Mouse blastocysts hatch in vitro by using a trypsin-like proteinase associated with cells of mural trophectoderm
AU - Perona, Rosario M.
AU - Wassarman, Paul M.
N1 - Funding Information:
We are grateful to all of the members of our laboratory for advice and constructive criticism throughout the course of this research. We thank Dr. Davor Solter and Dr. Barbara Knowles for a generous gift of rabbit anti-mouse spleen serum, Dr. Sid Strickland for valuable advice and discussions, and Fr. George Salzmann for suggesting the name strypsin. The research was supported in part by a grant from the National Institute of Child Health and Human Development (HD-12275) awarded to P.M.W. R.M.P. was supported in part by postdoctoral fellowships from the Juan March Foundation and the Fogarty International Center.
PY - 1986/3
Y1 - 1986/3
N2 - The mammalian blastocyst must hatch from its extracellular coat, or zona pellucida, to implant in the uterus and continue development normally. Results of experiments described here strongly suggest that a proteinase (74K Mr), called "strypsin," is directly involved in hatching of isolated mouse blastocysts in vitro. Strypsin is a trypsin-like proteinase, based on its substrate specificity and sensitivity to inhibitors, that is present in mouse blastocysts and exhibits certain properties characteristic of membrane-associated enzymes. Histochemical and autoradiographic evidence suggests that, prior to hatching of blastocysts, strypsin is found with cells of mural trophectoderm; not with polar trophectoderm or inner cell mass. Following hatching, strypsin is also found associated with empty zonae pellucidae, specifically at the opening through which the embryo emerged. These and other observations suggest that hatching of mouse blastocysts in vitro is initiated by limited proteolysis of the region of zona pellucida overlying mural trophectoderm.
AB - The mammalian blastocyst must hatch from its extracellular coat, or zona pellucida, to implant in the uterus and continue development normally. Results of experiments described here strongly suggest that a proteinase (74K Mr), called "strypsin," is directly involved in hatching of isolated mouse blastocysts in vitro. Strypsin is a trypsin-like proteinase, based on its substrate specificity and sensitivity to inhibitors, that is present in mouse blastocysts and exhibits certain properties characteristic of membrane-associated enzymes. Histochemical and autoradiographic evidence suggests that, prior to hatching of blastocysts, strypsin is found with cells of mural trophectoderm; not with polar trophectoderm or inner cell mass. Following hatching, strypsin is also found associated with empty zonae pellucidae, specifically at the opening through which the embryo emerged. These and other observations suggest that hatching of mouse blastocysts in vitro is initiated by limited proteolysis of the region of zona pellucida overlying mural trophectoderm.
UR - https://www.scopus.com/pages/publications/0022922269
U2 - 10.1016/0012-1606(86)90382-9
DO - 10.1016/0012-1606(86)90382-9
M3 - Article
C2 - 3514318
AN - SCOPUS:0022922269
SN - 0012-1606
VL - 114
SP - 42
EP - 52
JO - Developmental Biology
JF - Developmental Biology
IS - 1
ER -