Morphologic and Cellular Changes Induced by Selective Laser Trabeculoplasty

Meenakashi Gupta; Jae Young Heo; Haiyan Gong; Elliot Cha; Mark Latina; Douglas J. Rhee

doi:10.2147/OPTH.S342787

Morphologic and Cellular Changes Induced by Selective Laser Trabeculoplasty

Meenakashi Gupta, Jae Young Heo, Haiyan Gong, Elliot Cha, Mark Latina, Douglas J. Rhee

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Purpose: Selective laser trabeculoplasty is a safe and effective procedure for reducing IOP, but its mechanism of action is not fully elucidated. We evaluated the morphologic and cellular changes as well as DNA synthesis after SLT treatment of human trabecular meshwork (TM) tissue explants. Methods: Corneoscleral rim tissues that underwent SLT treatment were compared to control segments that had no laser treatment. Light microscopy (LM), transmission electron microscopy (TEM), and scanning electron microscopy (SEM) were used to assess cell morphology. The Click-iT 5-ethynyl-2’-deoxyuridine (EdU) imaging kit was used to compare DNA synthesis/cell proliferation with a confocal microscope. All tissues were assessed for vitality. Results: SLT treatment does not reveal notable cell damage in the juxtacanalicular (JCT) region, but mildly disrupts superficial trabecular beams and uveal TM, ablates TM endothelial cells from the undamaged beams as detected by both LM and TEM. This superficial destruction was not observed in some SLT treatment spots on higher magnification by SEM. SLT treatment increased mitotic activity and DNA synthesis near the lining of Schlemm’s canal after several days. Conclusion: SLT treatment disrupts endothelial cells in the corneoscleral TM and causes superficial ultrastructural changes to the uveal TM. SLT treatment also shows a trend towards dynamic time-dependent changes in (DNA synthesis) with an increase in mitotic activity at 7 days cell proliferation.

Original language	English
Pages (from-to)	1383-1390
Number of pages	8
Journal	Clinical Ophthalmology
Volume	16
DOIs	https://doi.org/10.2147/OPTH.S342787
State	Published - 2022
Externally published	Yes

Keywords

DNA synthesis
electron microscopy
glaucoma
selective laser trabeculoplasty

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10.2147/OPTH.S342787

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@article{04c59b902775469784f3cec6e40a88f8,

title = "Morphologic and Cellular Changes Induced by Selective Laser Trabeculoplasty",

abstract = "Purpose: Selective laser trabeculoplasty is a safe and effective procedure for reducing IOP, but its mechanism of action is not fully elucidated. We evaluated the morphologic and cellular changes as well as DNA synthesis after SLT treatment of human trabecular meshwork (TM) tissue explants. Methods: Corneoscleral rim tissues that underwent SLT treatment were compared to control segments that had no laser treatment. Light microscopy (LM), transmission electron microscopy (TEM), and scanning electron microscopy (SEM) were used to assess cell morphology. The Click-iT 5-ethynyl-2{\textquoteright}-deoxyuridine (EdU) imaging kit was used to compare DNA synthesis/cell proliferation with a confocal microscope. All tissues were assessed for vitality. Results: SLT treatment does not reveal notable cell damage in the juxtacanalicular (JCT) region, but mildly disrupts superficial trabecular beams and uveal TM, ablates TM endothelial cells from the undamaged beams as detected by both LM and TEM. This superficial destruction was not observed in some SLT treatment spots on higher magnification by SEM. SLT treatment increased mitotic activity and DNA synthesis near the lining of Schlemm{\textquoteright}s canal after several days. Conclusion: SLT treatment disrupts endothelial cells in the corneoscleral TM and causes superficial ultrastructural changes to the uveal TM. SLT treatment also shows a trend towards dynamic time-dependent changes in (DNA synthesis) with an increase in mitotic activity at 7 days cell proliferation.",

keywords = "DNA synthesis, electron microscopy, glaucoma, selective laser trabeculoplasty",

author = "Meenakashi Gupta and Heo, {Jae Young} and Haiyan Gong and Elliot Cha and Mark Latina and Rhee, {Douglas J.}",

note = "Funding Information: Declaration of interest for Corresponding author: Research Funding (to Institution): Allergan, Glaukos, Ivantis; Consultant: Aerie, Alcon, Allergan, Bausch and Lomb, Ivantis, pH-Pharma;Speakers Bureau: Aerie, Bausch and Lomb, Ivantis;Scientific Advisory Board: Ocular Therapeutix. Mr Jae Young Heo reports grants from National Eye Institute, grants from Research to Prevent Blindness, grants from Cleveland Eye Bank Foundation, grants from The Massachusetts Lions Eye Research Fund, during the conduct of the study. Dr Mark Latina reports a patent Selective Targeting of TM cells issued to Lumenis. The authors report no other conflicts in interest in this work. Funding Information: Supported by: National Eye Institute 5R01EY019654-02 (Rhee), R01EY022634 (Gong) and P30EY011373 (VSRC Core Grant), unrestricted funds from the Research to Prevent Blindness, unrestricted funds from the Cleveland Eye Bank Foundation and The Massachusetts Lions Eye Research Fund (Gong). Publisher Copyright: {\textcopyright} 2022 Gupta et al.",

year = "2022",

doi = "10.2147/OPTH.S342787",

language = "English",

volume = "16",

pages = "1383--1390",

journal = "Clinical Ophthalmology",

issn = "1177-5467",

publisher = "Dove Medical Press Ltd.",

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TY - JOUR

T1 - Morphologic and Cellular Changes Induced by Selective Laser Trabeculoplasty

AU - Gupta, Meenakashi

AU - Heo, Jae Young

AU - Gong, Haiyan

AU - Cha, Elliot

AU - Latina, Mark

AU - Rhee, Douglas J.

N1 - Funding Information: Declaration of interest for Corresponding author: Research Funding (to Institution): Allergan, Glaukos, Ivantis; Consultant: Aerie, Alcon, Allergan, Bausch and Lomb, Ivantis, pH-Pharma;Speakers Bureau: Aerie, Bausch and Lomb, Ivantis;Scientific Advisory Board: Ocular Therapeutix. Mr Jae Young Heo reports grants from National Eye Institute, grants from Research to Prevent Blindness, grants from Cleveland Eye Bank Foundation, grants from The Massachusetts Lions Eye Research Fund, during the conduct of the study. Dr Mark Latina reports a patent Selective Targeting of TM cells issued to Lumenis. The authors report no other conflicts in interest in this work. Funding Information: Supported by: National Eye Institute 5R01EY019654-02 (Rhee), R01EY022634 (Gong) and P30EY011373 (VSRC Core Grant), unrestricted funds from the Research to Prevent Blindness, unrestricted funds from the Cleveland Eye Bank Foundation and The Massachusetts Lions Eye Research Fund (Gong). Publisher Copyright: © 2022 Gupta et al.

PY - 2022

Y1 - 2022

N2 - Purpose: Selective laser trabeculoplasty is a safe and effective procedure for reducing IOP, but its mechanism of action is not fully elucidated. We evaluated the morphologic and cellular changes as well as DNA synthesis after SLT treatment of human trabecular meshwork (TM) tissue explants. Methods: Corneoscleral rim tissues that underwent SLT treatment were compared to control segments that had no laser treatment. Light microscopy (LM), transmission electron microscopy (TEM), and scanning electron microscopy (SEM) were used to assess cell morphology. The Click-iT 5-ethynyl-2’-deoxyuridine (EdU) imaging kit was used to compare DNA synthesis/cell proliferation with a confocal microscope. All tissues were assessed for vitality. Results: SLT treatment does not reveal notable cell damage in the juxtacanalicular (JCT) region, but mildly disrupts superficial trabecular beams and uveal TM, ablates TM endothelial cells from the undamaged beams as detected by both LM and TEM. This superficial destruction was not observed in some SLT treatment spots on higher magnification by SEM. SLT treatment increased mitotic activity and DNA synthesis near the lining of Schlemm’s canal after several days. Conclusion: SLT treatment disrupts endothelial cells in the corneoscleral TM and causes superficial ultrastructural changes to the uveal TM. SLT treatment also shows a trend towards dynamic time-dependent changes in (DNA synthesis) with an increase in mitotic activity at 7 days cell proliferation.

AB - Purpose: Selective laser trabeculoplasty is a safe and effective procedure for reducing IOP, but its mechanism of action is not fully elucidated. We evaluated the morphologic and cellular changes as well as DNA synthesis after SLT treatment of human trabecular meshwork (TM) tissue explants. Methods: Corneoscleral rim tissues that underwent SLT treatment were compared to control segments that had no laser treatment. Light microscopy (LM), transmission electron microscopy (TEM), and scanning electron microscopy (SEM) were used to assess cell morphology. The Click-iT 5-ethynyl-2’-deoxyuridine (EdU) imaging kit was used to compare DNA synthesis/cell proliferation with a confocal microscope. All tissues were assessed for vitality. Results: SLT treatment does not reveal notable cell damage in the juxtacanalicular (JCT) region, but mildly disrupts superficial trabecular beams and uveal TM, ablates TM endothelial cells from the undamaged beams as detected by both LM and TEM. This superficial destruction was not observed in some SLT treatment spots on higher magnification by SEM. SLT treatment increased mitotic activity and DNA synthesis near the lining of Schlemm’s canal after several days. Conclusion: SLT treatment disrupts endothelial cells in the corneoscleral TM and causes superficial ultrastructural changes to the uveal TM. SLT treatment also shows a trend towards dynamic time-dependent changes in (DNA synthesis) with an increase in mitotic activity at 7 days cell proliferation.

KW - DNA synthesis

KW - electron microscopy

KW - glaucoma

KW - selective laser trabeculoplasty

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U2 - 10.2147/OPTH.S342787

DO - 10.2147/OPTH.S342787

M3 - Article

AN - SCOPUS:85129946464

SN - 1177-5467

VL - 16

SP - 1383

EP - 1390

JO - Clinical Ophthalmology

JF - Clinical Ophthalmology

ER -

Morphologic and Cellular Changes Induced by Selective Laser Trabeculoplasty

Abstract

Keywords

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