Monoiodoglucagon: Synthesis, Purification by High Pressure Liquid Chromatography, and Characteristics as a Receptor Probe

Francisco J. Rojas; Theodore L. Swartz; Ravi Iyengar; Alan J. Garber; Lutz Birnbaumer

doi:10.1210/endo-113-2-711

Monoiodoglucagon: Synthesis, Purification by High Pressure Liquid Chromatography, and Characteristics as a Receptor Probe

Francisco J. Rojas
, Theodore L. Swartz
, Ravi Iyengar
, Alan J. Garber
, Lutz Birnbaumer

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

The newly synthesized [¹²⁵I] monoiodoglucagon is shown to activate adenylyl cyclase in liver membranes with an EC₅₀ between 5- and 8-fold lower than that of native glucagon. Further, it binds specifically to sites on liver plasma membranes that have the characteristics of glucagon receptors in terms of gua-nine nucleotide sensitivity and rates of reaction. It is suggested that [¹²⁵I-Tyr¹⁰]monoiodoglucagon is a suitable probe for studying structural and functional properties of glucagon receptors.

Original language	English
Pages (from-to)	711-719
Number of pages	9
Journal	Endocrinology
Volume	113
Issue number	2
DOIs	https://doi.org/10.1210/endo-113-2-711
State	Published - Aug 1983
Externally published	Yes

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title = "Monoiodoglucagon: Synthesis, Purification by High Pressure Liquid Chromatography, and Characteristics as a Receptor Probe",

abstract = "The newly synthesized [125I] monoiodoglucagon is shown to activate adenylyl cyclase in liver membranes with an EC50 between 5- and 8-fold lower than that of native glucagon. Further, it binds specifically to sites on liver plasma membranes that have the characteristics of glucagon receptors in terms of gua-nine nucleotide sensitivity and rates of reaction. It is suggested that [125I-Tyr10]monoiodoglucagon is a suitable probe for studying structural and functional properties of glucagon receptors.",

author = "Rojas, \{Francisco J.\} and Swartz, \{Theodore L.\} and Ravi Iyengar and Garber, \{Alan J.\} and Lutz Birnbaumer",

year = "1983",

month = aug,

doi = "10.1210/endo-113-2-711",

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T2 - Synthesis, Purification by High Pressure Liquid Chromatography, and Characteristics as a Receptor Probe

AU - Rojas, Francisco J.

AU - Swartz, Theodore L.

AU - Iyengar, Ravi

AU - Garber, Alan J.

AU - Birnbaumer, Lutz

PY - 1983/8

Y1 - 1983/8

N2 - The newly synthesized [125I] monoiodoglucagon is shown to activate adenylyl cyclase in liver membranes with an EC50 between 5- and 8-fold lower than that of native glucagon. Further, it binds specifically to sites on liver plasma membranes that have the characteristics of glucagon receptors in terms of gua-nine nucleotide sensitivity and rates of reaction. It is suggested that [125I-Tyr10]monoiodoglucagon is a suitable probe for studying structural and functional properties of glucagon receptors.

AB - The newly synthesized [125I] monoiodoglucagon is shown to activate adenylyl cyclase in liver membranes with an EC50 between 5- and 8-fold lower than that of native glucagon. Further, it binds specifically to sites on liver plasma membranes that have the characteristics of glucagon receptors in terms of gua-nine nucleotide sensitivity and rates of reaction. It is suggested that [125I-Tyr10]monoiodoglucagon is a suitable probe for studying structural and functional properties of glucagon receptors.

UR - https://www.scopus.com/pages/publications/0020556907

U2 - 10.1210/endo-113-2-711

DO - 10.1210/endo-113-2-711

M3 - Article

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AN - SCOPUS:0020556907

SN - 0013-7227

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JO - Endocrinology

JF - Endocrinology

IS - 2

ER -

Monoiodoglucagon: Synthesis, Purification by High Pressure Liquid Chromatography, and Characteristics as a Receptor Probe

Abstract

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