Monoiodoglucagon: Synthesis, Purification by High Pressure Liquid Chromatography, and Characteristics as a Receptor Probe

Francisco J. Rojas; Theodore L. Swartz; Ravi Iyengar; Alan J. Garber; Lutz Birnbaumer

doi:10.1210/endo-113-2-711

Monoiodoglucagon: Synthesis, Purification by High Pressure Liquid Chromatography, and Characteristics as a Receptor Probe

Francisco J. Rojas, Theodore L. Swartz, Ravi Iyengar, Alan J. Garber, Lutz Birnbaumer

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

The newly synthesized [¹²⁵I] monoiodoglucagon is shown to activate adenylyl cyclase in liver membranes with an EC₅₀ between 5- and 8-fold lower than that of native glucagon. Further, it binds specifically to sites on liver plasma membranes that have the characteristics of glucagon receptors in terms of gua-nine nucleotide sensitivity and rates of reaction. It is suggested that [¹²⁵I-Tyr¹⁰]monoiodoglucagon is a suitable probe for studying structural and functional properties of glucagon receptors.

Original language	English
Pages (from-to)	711-719
Number of pages	9
Journal	Endocrinology
Volume	113
Issue number	2
DOIs	https://doi.org/10.1210/endo-113-2-711
State	Published - Aug 1983
Externally published	Yes

Access to Document

10.1210/endo-113-2-711

Cite this

@article{0d7ae4b28d754fb292ab9ebbd6552165,

title = "Monoiodoglucagon: Synthesis, Purification by High Pressure Liquid Chromatography, and Characteristics as a Receptor Probe",

abstract = "The newly synthesized [125I] monoiodoglucagon is shown to activate adenylyl cyclase in liver membranes with an EC50 between 5- and 8-fold lower than that of native glucagon. Further, it binds specifically to sites on liver plasma membranes that have the characteristics of glucagon receptors in terms of gua-nine nucleotide sensitivity and rates of reaction. It is suggested that [125I-Tyr10]monoiodoglucagon is a suitable probe for studying structural and functional properties of glucagon receptors.",

author = "Rojas, {Francisco J.} and Swartz, {Theodore L.} and Ravi Iyengar and Garber, {Alan J.} and Lutz Birnbaumer",

year = "1983",

month = aug,

doi = "10.1210/endo-113-2-711",

language = "English",

volume = "113",

pages = "711--719",

journal = "Endocrinology",

issn = "0013-7227",

publisher = "Endocrine Society",

number = "2",

}

TY - JOUR

T1 - Monoiodoglucagon

T2 - Synthesis, Purification by High Pressure Liquid Chromatography, and Characteristics as a Receptor Probe

AU - Rojas, Francisco J.

AU - Swartz, Theodore L.

AU - Iyengar, Ravi

AU - Garber, Alan J.

AU - Birnbaumer, Lutz

PY - 1983/8

Y1 - 1983/8

N2 - The newly synthesized [125I] monoiodoglucagon is shown to activate adenylyl cyclase in liver membranes with an EC50 between 5- and 8-fold lower than that of native glucagon. Further, it binds specifically to sites on liver plasma membranes that have the characteristics of glucagon receptors in terms of gua-nine nucleotide sensitivity and rates of reaction. It is suggested that [125I-Tyr10]monoiodoglucagon is a suitable probe for studying structural and functional properties of glucagon receptors.

AB - The newly synthesized [125I] monoiodoglucagon is shown to activate adenylyl cyclase in liver membranes with an EC50 between 5- and 8-fold lower than that of native glucagon. Further, it binds specifically to sites on liver plasma membranes that have the characteristics of glucagon receptors in terms of gua-nine nucleotide sensitivity and rates of reaction. It is suggested that [125I-Tyr10]monoiodoglucagon is a suitable probe for studying structural and functional properties of glucagon receptors.

UR - http://www.scopus.com/inward/record.url?scp=0020556907&partnerID=8YFLogxK

U2 - 10.1210/endo-113-2-711

DO - 10.1210/endo-113-2-711

M3 - Article

C2 - 6307649

AN - SCOPUS:0020556907

SN - 0013-7227

VL - 113

SP - 711

EP - 719

JO - Endocrinology

JF - Endocrinology

IS - 2

ER -

Monoiodoglucagon: Synthesis, Purification by High Pressure Liquid Chromatography, and Characteristics as a Receptor Probe

Abstract

Access to Document

Fingerprint

Cite this