Abstract
1. 1. Six different monoclonal IgG mouse antibodies to heparin lyase I from Flavobacterium heparinum were prepared. 2. 2. The monoclonal antibodies were used to detect heparin lyases I, II and III by dot-blotting immunoassay and by Western blotting. 3. 3. Individual antibodies showed different reactivity toward the three heparin lyases. 4. 4. The reactivity of two of the monoclonal antibodies was destroyed by exposing heparin lyases to sodium dodecyl sulfate. 5. 5. The antibodies can be used to rapidly distinguish between the three heparin lyases.
Original language | English |
---|---|
Pages (from-to) | 331-336 |
Number of pages | 6 |
Journal | International Journal of Biochemistry |
Volume | 25 |
Issue number | 3 |
DOIs | |
State | Published - Mar 1993 |
Externally published | Yes |