Monoclonal antibodies prepared against heparin lyase I and their reactivity toward heparin lyase I, II and III

Kenan Gu, R. Erik Edens, Kenneth A. Jandik, Robert J. Linhardt

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

1. 1. Six different monoclonal IgG mouse antibodies to heparin lyase I from Flavobacterium heparinum were prepared. 2. 2. The monoclonal antibodies were used to detect heparin lyases I, II and III by dot-blotting immunoassay and by Western blotting. 3. 3. Individual antibodies showed different reactivity toward the three heparin lyases. 4. 4. The reactivity of two of the monoclonal antibodies was destroyed by exposing heparin lyases to sodium dodecyl sulfate. 5. 5. The antibodies can be used to rapidly distinguish between the three heparin lyases.

Original languageEnglish
Pages (from-to)331-336
Number of pages6
JournalInternational Journal of Biochemistry
Volume25
Issue number3
DOIs
StatePublished - Mar 1993
Externally publishedYes

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