TY - JOUR
T1 - Monoclonal antibodies directed against human tumor‐associated antigens cross‐react with Drosophila proteins in clusters
AU - Dubreuil, Ronald R.
AU - Souik, Paula
AU - Haines, G. Kenneth
AU - Radosevich, James A.
PY - 1994
Y1 - 1994
N2 - We reacted the Third International IASLC Workshop panel of monoclonal antibodies (MAbs) directed against human lung‐tumor‐associated epitopes with nitrocellulose blots of total proteins from adult fruit flies. Out of 63 MAbs tested, 9 showed a significant reaction with Drosophila proteins. Interestingly, in a double‐blind analysis, most of the positive reactions fell into clusters that parallel the antibody reactivities against human tissues. In light of our findings, it becomes possible to screen expression vector libraries in order to isolate Drosophila cDNA that may have homology with human tumor‐associated antigens.
AB - We reacted the Third International IASLC Workshop panel of monoclonal antibodies (MAbs) directed against human lung‐tumor‐associated epitopes with nitrocellulose blots of total proteins from adult fruit flies. Out of 63 MAbs tested, 9 showed a significant reaction with Drosophila proteins. Interestingly, in a double‐blind analysis, most of the positive reactions fell into clusters that parallel the antibody reactivities against human tissues. In light of our findings, it becomes possible to screen expression vector libraries in order to isolate Drosophila cDNA that may have homology with human tumor‐associated antigens.
UR - https://www.scopus.com/pages/publications/0028321937
U2 - 10.1002/ijc.2910570720
DO - 10.1002/ijc.2910570720
M3 - Article
C2 - 8194902
AN - SCOPUS:0028321937
SN - 0020-7136
VL - 57
SP - 96
EP - 97
JO - International Journal of Cancer
JF - International Journal of Cancer
IS - 8 S
ER -