TY - JOUR
T1 - Molecularly imprinted polypyrrole based sensor for the detection of SARS-CoV-2 spike glycoprotein
AU - Ratautaite, Vilma
AU - Boguzaite, Raimonda
AU - Brazys, Ernestas
AU - Ramanaviciene, Almira
AU - Ciplys, Evaldas
AU - Juozapaitis, Mindaugas
AU - Slibinskas, Rimantas
AU - Bechelany, Mikhael
AU - Ramanavicius, Arunas
N1 - Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2022/1/20
Y1 - 2022/1/20
N2 - This study describes the application of a polypyrrole-based sensor for the determination of SARS-CoV-2-S spike glycoprotein. The SARS-CoV-2-S spike glycoprotein is a spike protein of the coronavirus SARS-CoV-2 that recently caused the worldwide spread of COVID-19 disease. This study is dedicated to the development of an electrochemical determination method based on the application of molecularly imprinted polymer technology. The electrochemical sensor was designed by molecular imprinting of polypyrrole (Ppy) with SARS-CoV-2-S spike glycoprotein (MIP-Ppy). The electrochemical sensors with MIP-Ppy and with polypyrrole without imprints (NIP-Ppy) layers were electrochemically deposited on a platinum electrode surface by a sequence of potential pulses. The performance of polymer layers was evaluated by pulsed amperometric detection. According to the obtained results, a sensor based on MIP-Ppy is more sensitive to the SARS-CoV-2-S spike glycoprotein than a sensor based on NIP-Ppy. Also, the results demonstrate that the MIP-Ppy layer is more selectively interacting with SARS-CoV-2-S glycoprotein than with bovine serum albumin. This proves that molecularly imprinted MIP-Ppy-based sensors can be applied for the detection of SARS-CoV-2 virus proteins.
AB - This study describes the application of a polypyrrole-based sensor for the determination of SARS-CoV-2-S spike glycoprotein. The SARS-CoV-2-S spike glycoprotein is a spike protein of the coronavirus SARS-CoV-2 that recently caused the worldwide spread of COVID-19 disease. This study is dedicated to the development of an electrochemical determination method based on the application of molecularly imprinted polymer technology. The electrochemical sensor was designed by molecular imprinting of polypyrrole (Ppy) with SARS-CoV-2-S spike glycoprotein (MIP-Ppy). The electrochemical sensors with MIP-Ppy and with polypyrrole without imprints (NIP-Ppy) layers were electrochemically deposited on a platinum electrode surface by a sequence of potential pulses. The performance of polymer layers was evaluated by pulsed amperometric detection. According to the obtained results, a sensor based on MIP-Ppy is more sensitive to the SARS-CoV-2-S spike glycoprotein than a sensor based on NIP-Ppy. Also, the results demonstrate that the MIP-Ppy layer is more selectively interacting with SARS-CoV-2-S glycoprotein than with bovine serum albumin. This proves that molecularly imprinted MIP-Ppy-based sensors can be applied for the detection of SARS-CoV-2 virus proteins.
KW - COVID-19
KW - Conducting polymers
KW - Electrochemical determination of virus proteins
KW - Molecularly imprinted polymers (MIPs)
KW - Polypyrrole (Ppy)
KW - SARS-CoV-2 spike glycoprotein
KW - Thin layers
UR - https://www.scopus.com/pages/publications/85120639129
U2 - 10.1016/j.electacta.2021.139581
DO - 10.1016/j.electacta.2021.139581
M3 - Article
AN - SCOPUS:85120639129
SN - 0013-4686
VL - 403
JO - Electrochimica Acta
JF - Electrochimica Acta
M1 - 139581
ER -