TY - JOUR
T1 - Molecular inventory of faecal microflora in patients with Crohn's disease
AU - Mangin, Irène
AU - Bonnet, Régis
AU - Seksik, Philippe
AU - Rigottier-Gois, Lionel
AU - Sutren, Malène
AU - Bouhnik, Yoram
AU - Neut, Christel
AU - Collins, Matthew D.
AU - Colombel, Jean Frédéric
AU - Marteau, Philippe
AU - Doré, Joël
N1 - Funding Information:
This work was supported by grants from Association François Aupetit, Paris, Société Nationale Française de Gastroentérologie, Paris, Institut de Recherche sur les Maladies de l'Appareil Digestif (IRMAD), Laboratoires AstraZeneca, Rueil Malmaison, France, and the European Research Project FLAIR CT97-3035. We acknowledge Genoscope, Evry, France, for part of the sequencing.
PY - 2004/10/1
Y1 - 2004/10/1
N2 - Intestinal microbial community is involved in the pathogenesis of Crohn's disease, but knowledge of its potential abnormalities has been limited by the impossibility to grow many dominant intestinal bacteria. Using sequence analysis of randomly cloned bacterial 16S ribosomal DNA, the dominant faecal species from four Crohn's disease patients and four controls were compared. Whereas marked inter-individual differences were observed in the faecal microflora of patients, three remained distantly related to controls on the basis of their operational taxonomic unit composition. Bacteroides vulgatus and closely related organisms represented the only molecular species shared by all patients and exhibited an unusually high rate of occurrence. Escherichia coli clones were isolated only in two patients with ileocolonic Crohn's disease. Moreover, numerous clones belonged to phylogenetic groups or species that are commonly not dominant in the faecal microflora of healthy subjects: Pectinatus, Sutterella, Verrucomicrobium, Fusobacterium, Clostridium disporicum, Clostridium glycolicum, Clostridium ramosum, Clostridium innocuum and Clostridium perfringens.
AB - Intestinal microbial community is involved in the pathogenesis of Crohn's disease, but knowledge of its potential abnormalities has been limited by the impossibility to grow many dominant intestinal bacteria. Using sequence analysis of randomly cloned bacterial 16S ribosomal DNA, the dominant faecal species from four Crohn's disease patients and four controls were compared. Whereas marked inter-individual differences were observed in the faecal microflora of patients, three remained distantly related to controls on the basis of their operational taxonomic unit composition. Bacteroides vulgatus and closely related organisms represented the only molecular species shared by all patients and exhibited an unusually high rate of occurrence. Escherichia coli clones were isolated only in two patients with ileocolonic Crohn's disease. Moreover, numerous clones belonged to phylogenetic groups or species that are commonly not dominant in the faecal microflora of healthy subjects: Pectinatus, Sutterella, Verrucomicrobium, Fusobacterium, Clostridium disporicum, Clostridium glycolicum, Clostridium ramosum, Clostridium innocuum and Clostridium perfringens.
KW - 16S rRNA gene sequencing
KW - Crohn's disease
KW - Faecal bacterial diversity
UR - https://www.scopus.com/pages/publications/4644231021
U2 - 10.1016/j.femsec.2004.05.005
DO - 10.1016/j.femsec.2004.05.005
M3 - Article
C2 - 19712374
AN - SCOPUS:4644231021
SN - 0168-6496
VL - 50
SP - 25
EP - 36
JO - FEMS Microbiology Ecology
JF - FEMS Microbiology Ecology
IS - 1
ER -