Molecular determinants for antibody binding on group 1 house dust mite allergens

Maksymilian Chruszcz, Anna Pomés, Jill Glesner, Lisa D. Vailes, Tomasz Osinski, Przemyslaw J. Porebski, Karolina A. Majorek, Peter W. Heymann, Thomas A.E. Platts-Mills, Wladek Minor, Martin D. Chapman

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

House dust mites produce potent allergens, Der p 1 and Der f 1, that cause allergic sensitization and asthma. Der p 1 and Der f 1 are cysteine proteases that elicit IgE responses in 80% of mite-allergic subjects and have proinflammatory properties. Their antigenic structure is unknown. Here, we present crystal structures of natural Der p 1 andDer f 1 in complex with a monoclonal antibody, 4C1, which binds to a unique cross-reactive epitope on both allergens associated with IgE recognition. The 4C1 epitope is formed by almost identical amino acid sequences and contact residues. Mutations of the contact residues abrogate mAb 4C1 binding and reduce IgE antibody binding. These surface-exposed residues are molecular targets that can be exploited for development of recombinant allergen vaccines.

Original languageEnglish
Pages (from-to)7388-7398
Number of pages11
JournalJournal of Biological Chemistry
Volume287
Issue number10
DOIs
StatePublished - 2 Mar 2012
Externally publishedYes

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