Molecular cloning of the human gene SUVCC2 associated with mutagenesis following the induction of non-dimer DNA damages by solar UV radiation

A mutant cell line, DRP 512, sensitive to the induction of non-dimer DNA damages produced by solar UV radiation was derived from ICR 2A frog cells. In addition, the DRP 512 cells exhibited an abnormally high level of ouabain-resistant mutants after exposure to solar UV. A level of 1.1 mutants per 10⁶ survivors per kJ m^-2 was measured for ICR 2A whereas the yield was 4.2 mutants per 10⁶ survivors per kJ m^-2 for the solar-UV-sensitive cell line. The DRP 512 cells were transfected with human DNA and a secondary transformant obtained in which normal solar UV sensitivity and mutation induction were restored. DNA from this secondary transformant was used to construct a genomic DNA library from which a recombinant phage was isolated containing the human gene capable of restoring normal solar UV sensitivity and mutation induction to DRP 512. This gene has been designated SUVCC2.