MOLECULAR CLONING OF THE HUMAN GENE SUVCC1 ASSOCIATED WITH THE REPAIR OF NONDIMER DNA DAMAGE INDUCED BY SOLAR UV RADIATION

Barry S. Rosenstein, Charles A. Vaslet, Rebecca B. Rosenstein

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Abstract— A mutant cell line, DRP 287, sensitive to solar UV radiation and deficient in the repair of solar UV‐induced nondimer DNA damage, was derived from ICR 2A frog cells. These cells were transfected with human DNA and a secondary transformant obtained in which normal solar UV sensitivity was restored and the repair defect corrected. The DNA from this secondary transformant was used to construct a genomic DNA library from which a recombinant phage was isolated containing the human gene capable of restoring normal solar UV sensitivity and correcting the repair defect in the DRP 287 cells. This represents the first human gene which has been isolated that is specifically involved in the repair of nondimer DNA damage induced by solar UV radiation. It has been designated SUVCC1 to denote solar UV cross‐complementing gene number 1.

Original languageEnglish
Pages (from-to)142-148
Number of pages7
JournalPhotochemistry and Photobiology
Volume61
Issue number2
DOIs
StatePublished - Feb 1995
Externally publishedYes

Fingerprint

Dive into the research topics of 'MOLECULAR CLONING OF THE HUMAN GENE SUVCC1 ASSOCIATED WITH THE REPAIR OF NONDIMER DNA DAMAGE INDUCED BY SOLAR UV RADIATION'. Together they form a unique fingerprint.

Cite this