TY - JOUR
T1 - MOLECULAR CLONING OF THE HUMAN GENE SUVCC1 ASSOCIATED WITH THE REPAIR OF NONDIMER DNA DAMAGE INDUCED BY SOLAR UV RADIATION
AU - Rosenstein, Barry S.
AU - Vaslet, Charles A.
AU - Rosenstein, Rebecca B.
PY - 1995/2
Y1 - 1995/2
N2 - Abstract— A mutant cell line, DRP 287, sensitive to solar UV radiation and deficient in the repair of solar UV‐induced nondimer DNA damage, was derived from ICR 2A frog cells. These cells were transfected with human DNA and a secondary transformant obtained in which normal solar UV sensitivity was restored and the repair defect corrected. The DNA from this secondary transformant was used to construct a genomic DNA library from which a recombinant phage was isolated containing the human gene capable of restoring normal solar UV sensitivity and correcting the repair defect in the DRP 287 cells. This represents the first human gene which has been isolated that is specifically involved in the repair of nondimer DNA damage induced by solar UV radiation. It has been designated SUVCC1 to denote solar UV cross‐complementing gene number 1.
AB - Abstract— A mutant cell line, DRP 287, sensitive to solar UV radiation and deficient in the repair of solar UV‐induced nondimer DNA damage, was derived from ICR 2A frog cells. These cells were transfected with human DNA and a secondary transformant obtained in which normal solar UV sensitivity was restored and the repair defect corrected. The DNA from this secondary transformant was used to construct a genomic DNA library from which a recombinant phage was isolated containing the human gene capable of restoring normal solar UV sensitivity and correcting the repair defect in the DRP 287 cells. This represents the first human gene which has been isolated that is specifically involved in the repair of nondimer DNA damage induced by solar UV radiation. It has been designated SUVCC1 to denote solar UV cross‐complementing gene number 1.
UR - http://www.scopus.com/inward/record.url?scp=0029240180&partnerID=8YFLogxK
U2 - 10.1111/j.1751-1097.1995.tb03952.x
DO - 10.1111/j.1751-1097.1995.tb03952.x
M3 - Article
C2 - 7899503
AN - SCOPUS:0029240180
SN - 0031-8655
VL - 61
SP - 142
EP - 148
JO - Photochemistry and Photobiology
JF - Photochemistry and Photobiology
IS - 2
ER -