Molecular cloning of human brain glutamate/aspartate transporter II

P. Shashidharan, Ian Wittenberg, Andreas Plaitakis

Research output: Contribution to journalArticlepeer-review

76 Scopus citations

Abstract

Glutamate transporters are membrane-bound proteins which are localized in glial cells and/or pre-synaptic glutamatergic nerve endings and are essential for the removal and termination of action of synaptic glutamate. Several cDNAs encoding glutamate transporters have been isolated from mammalian tissues, including human cerebellum. Here, we screened cDNA libraries derived from human brain stem and cerebellum, and isolated a novel cDNA that encodes for a glutamate transporter. This cDNA predicts a protein which contains 565 amino acids and is homologous to a rat brain Na+-dependent glutamate/aspartate transporter. The new cDNA is expressed in brain and is structurally distinct from the previously reported human glutamate transporter cDNA.

Original languageEnglish
Pages (from-to)393-396
Number of pages4
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1191
Issue number2
DOIs
StatePublished - 11 May 1994
Externally publishedYes

Keywords

  • Cloning
  • Glutamate transporter
  • Human brain stem
  • Sequence
  • cDNA

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