Molecular cloning of cDNA encoding the 110 kDa and 21 kDa regulatory subunits of smooth muscle protein phosphatase 1M

Yu Hua Chen; Mao Xiang Chen; Dario R. Alessi; David G. Campbell; Catherine Shanahan; Philip Cohen; Patricia T.W. Cohen

doi:10.1016/0014-5793(94)01231-8

Molecular cloning of cDNA encoding the 110 kDa and 21 kDa regulatory subunits of smooth muscle protein phosphatase 1M

Yu Hua Chen
, Mao Xiang Chen
, Dario R. Alessi
, David G. Campbell
, Catherine Shanahan
, Philip Cohen
, Patricia T.W. Cohen

Research output: Contribution to journal › Article › peer-review

132 Scopus citations

Abstract

The structures of the M₁₁₀ and M₂₁ regulatory subunits of protein phosphatase-1M, the major enzyme which dephosphorylates myosin in smooth muscle, have been deduced from cloned cDNAs. The N-terminus of the M₁₁₀ subunit from rat aorta contains seven ankyrin repeats, while the C-terminus of the M₂₁ subunit from chicken gizzard contains a leucine zipper motif. The M₁₁₀ subunit is expressed in two different forms which differ in their C-terminal sequences. One of these is highly homologous to the whole of the M₂₁ subunit.

Original language	English
Pages (from-to)	51-55
Number of pages	5
Journal	FEBS Letters
Volume	356
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(94)01231-8
State	Published - 14 Dec 1994
Externally published	Yes

Keywords

Ankyrin repeat
Leucine zipper
Myosin
Protein phosphatase
Smooth muscle
cDNA sequence

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10.1016/0014-5793(94)01231-8

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title = "Molecular cloning of cDNA encoding the 110 kDa and 21 kDa regulatory subunits of smooth muscle protein phosphatase 1M",

abstract = "The structures of the M110 and M21 regulatory subunits of protein phosphatase-1M, the major enzyme which dephosphorylates myosin in smooth muscle, have been deduced from cloned cDNAs. The N-terminus of the M110 subunit from rat aorta contains seven ankyrin repeats, while the C-terminus of the M21 subunit from chicken gizzard contains a leucine zipper motif. The M110 subunit is expressed in two different forms which differ in their C-terminal sequences. One of these is highly homologous to the whole of the M21 subunit.",

keywords = "Ankyrin repeat, Leucine zipper, Myosin, Protein phosphatase, Smooth muscle, cDNA sequence",

author = "Chen, \{Yu Hua\} and Chen, \{Mao Xiang\} and Alessi, \{Dario R.\} and Campbell, \{David G.\} and Catherine Shanahan and Philip Cohen and Cohen, \{Patricia T.W.\}",

year = "1994",

month = dec,

day = "14",

doi = "10.1016/0014-5793(94)01231-8",

language = "English",

volume = "356",

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journal = "FEBS Letters",

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T1 - Molecular cloning of cDNA encoding the 110 kDa and 21 kDa regulatory subunits of smooth muscle protein phosphatase 1M

AU - Chen, Yu Hua

AU - Chen, Mao Xiang

AU - Alessi, Dario R.

AU - Campbell, David G.

AU - Shanahan, Catherine

AU - Cohen, Philip

AU - Cohen, Patricia T.W.

PY - 1994/12/14

Y1 - 1994/12/14

N2 - The structures of the M110 and M21 regulatory subunits of protein phosphatase-1M, the major enzyme which dephosphorylates myosin in smooth muscle, have been deduced from cloned cDNAs. The N-terminus of the M110 subunit from rat aorta contains seven ankyrin repeats, while the C-terminus of the M21 subunit from chicken gizzard contains a leucine zipper motif. The M110 subunit is expressed in two different forms which differ in their C-terminal sequences. One of these is highly homologous to the whole of the M21 subunit.

AB - The structures of the M110 and M21 regulatory subunits of protein phosphatase-1M, the major enzyme which dephosphorylates myosin in smooth muscle, have been deduced from cloned cDNAs. The N-terminus of the M110 subunit from rat aorta contains seven ankyrin repeats, while the C-terminus of the M21 subunit from chicken gizzard contains a leucine zipper motif. The M110 subunit is expressed in two different forms which differ in their C-terminal sequences. One of these is highly homologous to the whole of the M21 subunit.

KW - Ankyrin repeat

KW - Leucine zipper

KW - Myosin

KW - Protein phosphatase

KW - Smooth muscle

KW - cDNA sequence

UR - https://www.scopus.com/pages/publications/0028114606

U2 - 10.1016/0014-5793(94)01231-8

DO - 10.1016/0014-5793(94)01231-8

M3 - Article

C2 - 7988720

AN - SCOPUS:0028114606

SN - 0014-5793

VL - 356

SP - 51

EP - 55

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Molecular cloning of cDNA encoding the 110 kDa and 21 kDa regulatory subunits of smooth muscle protein phosphatase 1M

Abstract

Keywords

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