Molecular cloning and expression of two small leucine-rich proteoglycan (SLRP) genes, dspg3l and optcl, in zebrafish

Weibin Zhou; Komei Shirabe; John Y. Kuwada

doi:10.1016/j.modgep.2005.11.003

Molecular cloning and expression of two small leucine-rich proteoglycan (SLRP) genes, dspg3l and optcl, in zebrafish

Weibin Zhou
, Komei Shirabe
, John Y. Kuwada

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Epiphycan (DSPG3) and opticin are two class III small leucine-rich proteoglycans (SLRP). We isolated two zebrafish cDNAs, dspg3l and optcl, that encode proteins homologous to epiphycan and opticin in other vertebrates. Like epiphycans in other species, dspg3l is exclusively expressed in the developing notochord and cartilage. optcl is expressed transiently in the developing nervous system, eyes and somites much like opticin. The zebrafish dspg3l and optcl genes are located in linkage group 4 and 11, respectively. The genomic locations for both genes in zebrafish are syntenic with the genomic locations of dspg3 and opticin (optc) in human and mouse. Synteny and the expression patterns of these genes suggest that the dspg3l and optcl are the orthologs to the mammalian dspg3 and optc genes, respectively.

Original language	English
Pages (from-to)	482-488
Number of pages	7
Journal	Gene Expression Patterns
Volume	6
Issue number	5
DOIs	https://doi.org/10.1016/j.modgep.2005.11.003
State	Published - Jun 2006
Externally published	Yes

Keywords

Cartilage
Dermatan sulphate proteoglycan 3
Dspg3
Epiphycan
Floor plate
LRR
Lens
Leucine-rich repeat
MHB
Midbrain hindbrain boundary
Optic vesicle
Opticin
Otic vesicle
PG-Lb
Proteoglycan
SLRP
Somite
Spinal cord
Zebrafish

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@article{99f54356a00d46afae70f640583b16f0,

title = "Molecular cloning and expression of two small leucine-rich proteoglycan (SLRP) genes, dspg3l and optcl, in zebrafish",

abstract = "Epiphycan (DSPG3) and opticin are two class III small leucine-rich proteoglycans (SLRP). We isolated two zebrafish cDNAs, dspg3l and optcl, that encode proteins homologous to epiphycan and opticin in other vertebrates. Like epiphycans in other species, dspg3l is exclusively expressed in the developing notochord and cartilage. optcl is expressed transiently in the developing nervous system, eyes and somites much like opticin. The zebrafish dspg3l and optcl genes are located in linkage group 4 and 11, respectively. The genomic locations for both genes in zebrafish are syntenic with the genomic locations of dspg3 and opticin (optc) in human and mouse. Synteny and the expression patterns of these genes suggest that the dspg3l and optcl are the orthologs to the mammalian dspg3 and optc genes, respectively.",

keywords = "Cartilage, Dermatan sulphate proteoglycan 3, Dspg3, Epiphycan, Floor plate, LRR, Lens, Leucine-rich repeat, MHB, Midbrain hindbrain boundary, Optic vesicle, Opticin, Otic vesicle, PG-Lb, Proteoglycan, SLRP, Somite, Spinal cord, Zebrafish",

author = "Weibin Zhou and Komei Shirabe and Kuwada, \{John Y.\}",

note = "Funding Information: We appreciate Dr Christine Thisse and Dr Bernard Thisse for providing the cDNA clone. Research was supported by a grant from NINDS to JYK.",

year = "2006",

month = jun,

doi = "10.1016/j.modgep.2005.11.003",

language = "English",

volume = "6",

pages = "482--488",

journal = "Gene Expression Patterns",

issn = "1567-133X",

publisher = "Elsevier B.V.",

number = "5",

}

TY - JOUR

T1 - Molecular cloning and expression of two small leucine-rich proteoglycan (SLRP) genes, dspg3l and optcl, in zebrafish

AU - Zhou, Weibin

AU - Shirabe, Komei

AU - Kuwada, John Y.

N1 - Funding Information: We appreciate Dr Christine Thisse and Dr Bernard Thisse for providing the cDNA clone. Research was supported by a grant from NINDS to JYK.

PY - 2006/6

Y1 - 2006/6

N2 - Epiphycan (DSPG3) and opticin are two class III small leucine-rich proteoglycans (SLRP). We isolated two zebrafish cDNAs, dspg3l and optcl, that encode proteins homologous to epiphycan and opticin in other vertebrates. Like epiphycans in other species, dspg3l is exclusively expressed in the developing notochord and cartilage. optcl is expressed transiently in the developing nervous system, eyes and somites much like opticin. The zebrafish dspg3l and optcl genes are located in linkage group 4 and 11, respectively. The genomic locations for both genes in zebrafish are syntenic with the genomic locations of dspg3 and opticin (optc) in human and mouse. Synteny and the expression patterns of these genes suggest that the dspg3l and optcl are the orthologs to the mammalian dspg3 and optc genes, respectively.

AB - Epiphycan (DSPG3) and opticin are two class III small leucine-rich proteoglycans (SLRP). We isolated two zebrafish cDNAs, dspg3l and optcl, that encode proteins homologous to epiphycan and opticin in other vertebrates. Like epiphycans in other species, dspg3l is exclusively expressed in the developing notochord and cartilage. optcl is expressed transiently in the developing nervous system, eyes and somites much like opticin. The zebrafish dspg3l and optcl genes are located in linkage group 4 and 11, respectively. The genomic locations for both genes in zebrafish are syntenic with the genomic locations of dspg3 and opticin (optc) in human and mouse. Synteny and the expression patterns of these genes suggest that the dspg3l and optcl are the orthologs to the mammalian dspg3 and optc genes, respectively.

KW - Cartilage

KW - Dermatan sulphate proteoglycan 3

KW - Dspg3

KW - Epiphycan

KW - Floor plate

KW - LRR

KW - Lens

KW - Leucine-rich repeat

KW - MHB

KW - Midbrain hindbrain boundary

KW - Optic vesicle

KW - Opticin

KW - Otic vesicle

KW - PG-Lb

KW - Proteoglycan

KW - SLRP

KW - Somite

KW - Spinal cord

KW - Zebrafish

UR - https://www.scopus.com/pages/publications/33744918528

U2 - 10.1016/j.modgep.2005.11.003

DO - 10.1016/j.modgep.2005.11.003

M3 - Article

C2 - 16458084

AN - SCOPUS:33744918528

SN - 1567-133X

VL - 6

SP - 482

EP - 488

JO - Gene Expression Patterns

JF - Gene Expression Patterns

IS - 5

ER -

Molecular cloning and expression of two small leucine-rich proteoglycan (SLRP) genes, dspg3l and optcl, in zebrafish

Abstract

Keywords

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