Molecular cloning and expression of phospholipase C epsilon 1 in zebrafish

Weibin Zhou, Friedhelm Hildebrandt

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Phospholipase C (PLC) is a key enzyme that generates inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG), two second messengers in signal transduction. Phospholipase Cε (PLCE1) is a unique member of the PLC family in that it carries both RasGEF and Ras-associating (RA) domains and may serve as an activator and an effector of small GTPases. Recently, mutations in PLCE1 have been associated with early-onset nephrotic syndrome. We have identified the zebrafish ortholog of PLCE1 and characterized its expression pattern in zebrafish embryos by in situ hybridization. Zebrafish plce1 gene encodes a protein of 2248 amino acids that shares 56% identity with mammalian PLCε. During zebrafish embryonic development, plce1 is expressed in a dynamic pattern in a variety of organs, such as brain, muscle, liver, and pronephric glomeruli. Our results suggest that zebrafish may be used as a model organism to address the function of PLCε during the development of these organs.

Original languageEnglish
Pages (from-to)282-288
Number of pages7
JournalGene Expression Patterns
Volume9
Issue number5
DOIs
StatePublished - Jun 2009
Externally publishedYes

Keywords

  • Glomerulus
  • Horizontal myoseptum
  • Muscle pioneer cells
  • Nephrotic syndrome
  • PLCE1
  • Phospholipase
  • Pronephros
  • Rhombomere
  • Zebrafish

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