Abstract
Objective: To investigate the homology and resistant mechanism of vancomycin-resistant Enterococci (VRE) isolates. Methods; A total of 9 VRE isolates were collected from 2006 to 2007 at PUMC hospital. The susceptibility of these isolates to 10 different antibiotics including vancomycin was tested by E-test. These strains were processed by brain heart infusion agar screening in the presence of vancomycin (6μg/ml), and were analyzed for genotypic characteristics using the multiplex PCR. The homology of the isolates was determined by pulsed-field gel eleetrophoresis (PFGE). Results; All the 9 VRE isolates were identified as Enterococci faecium. The visual analysis of PFGE patterns revealed 6 different PFGE types. The vanA gene was confirmed by PCR and sequencing in 9 VRE isolates, which were consistent between phenotype and genotype for glycopeptides resistance. Conclusions: Only vanA genotype was detected in PUMC hospital. Clonal dissemination, horizontal gene transfer, and the selective pressure of antimicrobial agents may contribute to the increase of VRE.
| Original language | English |
|---|---|
| Pages (from-to) | 521-524 |
| Number of pages | 4 |
| Journal | Acta Academiae Medicinae Sinicae |
| Volume | 30 |
| Issue number | 5 |
| DOIs | |
| State | Published - Oct 2008 |
| Externally published | Yes |
Keywords
- Enterococci
- Genotype
- Homology
- Vancomycin-resistant
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