TY - JOUR
T1 - Molecular characterization of two strains of shope fibroma virus
AU - Berkowitz, Elchonon M.
AU - Pogo, Beatriz G.T.
N1 - Funding Information:
wae supported by Grant CA 29262 from Institutes of Health and by the Che-Foundation Inc.
PY - 1985/4/30
Y1 - 1985/4/30
N2 - An isolate of Shope fibroma virus (SFV), designated Indiana (SFV-I), was previously described to be tumorigenic in vivo as SFV, cytocidal in vitro as the orthopoxviruses (vaccinia, rabbitpox, etc.), and to share antigenic determinants with SFV and vaccinia. The genetic relatedness of SFV-I to SFV and vaccinia was studied by means of Southern blotting and hybridization. The results indicated that SFV-I shares extensive DNA homology with vaccinia, but few common sequences with SFV. By contrast, SFV and vaccinia show no sequence homology. These findings suggest that SFV-I is an orthopoxvirus which carries some genetic information from the leporipoxviruses. Recombinants between two genera of poxviruses have not been reported before.
AB - An isolate of Shope fibroma virus (SFV), designated Indiana (SFV-I), was previously described to be tumorigenic in vivo as SFV, cytocidal in vitro as the orthopoxviruses (vaccinia, rabbitpox, etc.), and to share antigenic determinants with SFV and vaccinia. The genetic relatedness of SFV-I to SFV and vaccinia was studied by means of Southern blotting and hybridization. The results indicated that SFV-I shares extensive DNA homology with vaccinia, but few common sequences with SFV. By contrast, SFV and vaccinia show no sequence homology. These findings suggest that SFV-I is an orthopoxvirus which carries some genetic information from the leporipoxviruses. Recombinants between two genera of poxviruses have not been reported before.
UR - http://www.scopus.com/inward/record.url?scp=0022283437&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(85)90354-X
DO - 10.1016/0042-6822(85)90354-X
M3 - Article
C2 - 2997996
AN - SCOPUS:0022283437
SN - 0042-6822
VL - 142
SP - 437
EP - 440
JO - Virology
JF - Virology
IS - 2
ER -