Modulation of neuronal nitric oxide release by soluble guanylyl cyclase in guinea pig colon

K. Hallén; C. Olgart; L. E. Gustafsson; N. P. Wiklund

doi:10.1006/bbrc.2001.4241

Modulation of neuronal nitric oxide release by soluble guanylyl cyclase in guinea pig colon

K. Hallén, C. Olgart, L. E. Gustafsson, N. P. Wiklund

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Peripheral autonomic neurones release nitric oxide (NO) upon nerve activation. However, the regulation of neuronal NO formation is poorly understood. We used the cyclic guanosine 3′,5′-monophosphate (cGMP) analogue 8-Br-cGMP, the soluble guanylyl cyclase (sGC) stimulator YC-1, the phosphodiesterase inhibitor zaprinast and the sGC inhibitor ODQ to study whether the sGC/cGMP pathway is involved in regulation of neuronal NO release in nerve plexus-containing smooth muscle preparations from guinea pig colon. Electrical stimulation of the preparation evoked release of NO/NO₂^-. In the presence of 8-Br-cGMP, YC-1 and zaprinast (all at 10^-4M) the NO/NO₂^-- release increased to 152 ± 16% (P < 0.05), 164 ± 37% (P < 0.05) and 290 ± 67% (P < 0.05) of controls, respectively. Conversely, ODQ (10^-5M) decreased the evoked release of NO/NO₂^- to 49 ± 7% (P < 0.05) of controls. Our data suggest that the sGC/cGMP pathway modulates NO release. Thus it is likely that NO exerts a positive feedback on its own release from peripheral autonomic neurones.

Original language	English
Pages (from-to)	1130-1134
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	280
Issue number	4
DOIs	https://doi.org/10.1006/bbrc.2001.4241
State	Published - 2001
Externally published	Yes

Keywords

Autonomic neurotransmission
Cyclic guanosine 3′,5′-monophosphate (cGMP)
Nitric oxide (NO)
Nitrite (NO)
ODQ
Phosphodiesterase (PDE)
Soluble guanylyl cyclase (sGC)
YC-1
Zaprinast

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10.1006/bbrc.2001.4241

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@article{405375d690c54620915433295c5bb1ce,

title = "Modulation of neuronal nitric oxide release by soluble guanylyl cyclase in guinea pig colon",

abstract = "Peripheral autonomic neurones release nitric oxide (NO) upon nerve activation. However, the regulation of neuronal NO formation is poorly understood. We used the cyclic guanosine 3′,5′-monophosphate (cGMP) analogue 8-Br-cGMP, the soluble guanylyl cyclase (sGC) stimulator YC-1, the phosphodiesterase inhibitor zaprinast and the sGC inhibitor ODQ to study whether the sGC/cGMP pathway is involved in regulation of neuronal NO release in nerve plexus-containing smooth muscle preparations from guinea pig colon. Electrical stimulation of the preparation evoked release of NO/NO2-. In the presence of 8-Br-cGMP, YC-1 and zaprinast (all at 10-4M) the NO/NO2-- release increased to 152 ± 16% (P < 0.05), 164 ± 37% (P < 0.05) and 290 ± 67% (P < 0.05) of controls, respectively. Conversely, ODQ (10-5M) decreased the evoked release of NO/NO2- to 49 ± 7% (P < 0.05) of controls. Our data suggest that the sGC/cGMP pathway modulates NO release. Thus it is likely that NO exerts a positive feedback on its own release from peripheral autonomic neurones.",

keywords = "Autonomic neurotransmission, Cyclic guanosine 3′,5′-monophosphate (cGMP), Nitric oxide (NO), Nitrite (NO), ODQ, Phosphodiesterase (PDE), Soluble guanylyl cyclase (sGC), YC-1, Zaprinast",

author = "K. Hall{\'e}n and C. Olgart and Gustafsson, {L. E.} and Wiklund, {N. P.}",

note = "Funding Information: This work was supported by Gunvor och Josef An{\'e}rs stiftelse, Stiftelsen Johanna Hagstrand och Sigfrid Linn{\'e}rs minne, Swedish Society for Medical Research, the Swedish MRC Grants 11199 and 7919, and the Karolinska Institute.",

year = "2001",

doi = "10.1006/bbrc.2001.4241",

language = "English",

volume = "280",

pages = "1130--1134",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

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number = "4",

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TY - JOUR

T1 - Modulation of neuronal nitric oxide release by soluble guanylyl cyclase in guinea pig colon

AU - Hallén, K.

AU - Olgart, C.

AU - Gustafsson, L. E.

AU - Wiklund, N. P.

N1 - Funding Information: This work was supported by Gunvor och Josef Anérs stiftelse, Stiftelsen Johanna Hagstrand och Sigfrid Linnérs minne, Swedish Society for Medical Research, the Swedish MRC Grants 11199 and 7919, and the Karolinska Institute.

PY - 2001

Y1 - 2001

N2 - Peripheral autonomic neurones release nitric oxide (NO) upon nerve activation. However, the regulation of neuronal NO formation is poorly understood. We used the cyclic guanosine 3′,5′-monophosphate (cGMP) analogue 8-Br-cGMP, the soluble guanylyl cyclase (sGC) stimulator YC-1, the phosphodiesterase inhibitor zaprinast and the sGC inhibitor ODQ to study whether the sGC/cGMP pathway is involved in regulation of neuronal NO release in nerve plexus-containing smooth muscle preparations from guinea pig colon. Electrical stimulation of the preparation evoked release of NO/NO2-. In the presence of 8-Br-cGMP, YC-1 and zaprinast (all at 10-4M) the NO/NO2-- release increased to 152 ± 16% (P < 0.05), 164 ± 37% (P < 0.05) and 290 ± 67% (P < 0.05) of controls, respectively. Conversely, ODQ (10-5M) decreased the evoked release of NO/NO2- to 49 ± 7% (P < 0.05) of controls. Our data suggest that the sGC/cGMP pathway modulates NO release. Thus it is likely that NO exerts a positive feedback on its own release from peripheral autonomic neurones.

AB - Peripheral autonomic neurones release nitric oxide (NO) upon nerve activation. However, the regulation of neuronal NO formation is poorly understood. We used the cyclic guanosine 3′,5′-monophosphate (cGMP) analogue 8-Br-cGMP, the soluble guanylyl cyclase (sGC) stimulator YC-1, the phosphodiesterase inhibitor zaprinast and the sGC inhibitor ODQ to study whether the sGC/cGMP pathway is involved in regulation of neuronal NO release in nerve plexus-containing smooth muscle preparations from guinea pig colon. Electrical stimulation of the preparation evoked release of NO/NO2-. In the presence of 8-Br-cGMP, YC-1 and zaprinast (all at 10-4M) the NO/NO2-- release increased to 152 ± 16% (P < 0.05), 164 ± 37% (P < 0.05) and 290 ± 67% (P < 0.05) of controls, respectively. Conversely, ODQ (10-5M) decreased the evoked release of NO/NO2- to 49 ± 7% (P < 0.05) of controls. Our data suggest that the sGC/cGMP pathway modulates NO release. Thus it is likely that NO exerts a positive feedback on its own release from peripheral autonomic neurones.

KW - Autonomic neurotransmission

KW - Cyclic guanosine 3′,5′-monophosphate (cGMP)

KW - Nitric oxide (NO)

KW - Nitrite (NO)

KW - ODQ

KW - Phosphodiesterase (PDE)

KW - Soluble guanylyl cyclase (sGC)

KW - YC-1

KW - Zaprinast

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U2 - 10.1006/bbrc.2001.4241

DO - 10.1006/bbrc.2001.4241

M3 - Article

C2 - 11162644

AN - SCOPUS:0034817341

SN - 0006-291X

VL - 280

SP - 1130

EP - 1134

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Modulation of neuronal nitric oxide release by soluble guanylyl cyclase in guinea pig colon

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Keywords

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