Modified methicillin-resistant Staphylococcus aureus detected in neonatal intensive care patients

Melissa R. Gitman, Bremy Alburquerque, Marilyn Chung, Adriana Van De Guchte, Mitchell J. Sullivan, Ajay Obla, Jose Polanco, Irina Oussenko, Melissa L. Smith, Flora Samaroo, Debbie Barackman, Deena R. Altman, Emilia Mia Sordillo, Harm Van Bakel

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Objectives: As part of an active MRSA surveillance programme in our neonatal ICU, we identified nares surveillance cultures from two infants that displayed heterogeneity in methicillin resistance between isolated subclones that lacked mecA and mecC. Methods: The underlying mechanism for the modified Staphylococcus aureus (MODSA) methicillin-resistance phenotype was investigated by WGS. Results: Comparison of finished-quality genomes of four MODSA and four MSSA subclones demonstrated that the resistance changes were associated with unique truncating mutations in the gene encoding the cyclic diadenosine monophosphate phosphodiesterase enzyme GdpP or a non-synonymous substitution in the gene encoding PBP2. Conclusions: These two cases highlight the difficulty in identifying non-mecA, non-mecC-mediated MRSA isolates in the clinical microbiology laboratory, which leads to difficulties in implementing appropriate therapy and infection control measures.

Original languageEnglish
Pages (from-to)2774-2777
Number of pages4
JournalJournal of Antimicrobial Chemotherapy
Volume76
Issue number11
DOIs
StatePublished - 1 Nov 2021

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