Modification of the composition of polycystin-1 multiprotein complexes by calcium and tyrosine phosphorylation

Lin Geng, Christopher R. Burrow, Hsi Ping Li, Patricia D. Wilson

Research output: Contribution to journalArticlepeer-review

90 Scopus citations

Abstract

Mutations in the PKD1 gene are responsible for >85% of autosomal dominant polycystic kidney disease (ADPKD). The protein product of PKD1, polycystin-1, is a large, modular membrane protein, with putative ligand-binding motifs in the extracelluar N-terminal portion, 9-11 transmembrane domains and an intracellular C-terminal portion with phosphorylation sites. A role for polycystin-1 as a cell surface receptor involved in cell-matrix and cell-cell interactions has been proposed. In this study, we have analyzed polycystin-1 and associated protein distribution in normal human epithelial cells and examined the role of cell-matrix versus cell-cell interactions in regulation of the assembly of polycystin-1 multiprotein complexes. Immunocytochemistry, sucrose density gradient sedimentation, co-immunoprecipitation analyses and in vitro binding assays have shown that polycystin-1 associates with the focal adhesion proteins talin, vinculin, p130Cas, FAK, α-actinin, paxillin and pp60c-src in subconfluent normal human fetal collecting tubule (HFCT) epithelia when cell-matrix interactions predominate. Polycystin-1 also forms higher S value complexes with the cell-cell adherens junction proteins E-cadherin, β- and γ-catenins in confluent cultures when cell-cell interactions are predominant. Polycystin-1 multiprotein complexes can be disrupted by cytochalasin D but not by colchicine, suggesting involvement of the actin cytoskeleton. Although inhibition of tyrosine phosphorylation by tyrphostin inhibits polycystin-1-FAK interactions, E-cadherin interactions are enhanced. High calcium treatment also increases polycystin-1-E-cadherin interactions. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)21-35
Number of pages15
JournalBiochimica et Biophysica Acta - Molecular Basis of Disease
Volume1535
Issue number1
DOIs
StatePublished - 15 Dec 2000

Keywords

  • Actin binding protein
  • Calcium
  • Focal adhesion
  • Focal adhesion kinase
  • Tyrosine phosphorylation
  • β-Catenin

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