Modification of catalytic properties of chicken liver fructose 1,6-bisphosphatase by allicin

J. Han, L. Lawson, T. C. Chu, D. Potter, G. Han, P. Han

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The activity of chicken liver fructose 1,6-bisphosphatase increases dramatically after incubation with allicin, a major biologically active compound produced by garlic. Activation is more pronounced when the enzyme is assayed with Mn2+ than Mg2+. Maximum activation is accompanied by the disappearance of 4 highly reactive sulfhydryl groups per molecule of enzyme. This modification also leads to loss of activation by K+, and reduced sensitivity to inhibition by AMP, fructose 2,6-bisphosphate, and high concentration of fructose 1,6-bisphosphate. All the altered properties induced by allicin can be reversed by dithiothreitol or tris(2-carboxyethyl)phosphine, the latter being much more effective.

Original languageEnglish
Pages (from-to)1007-1015
Number of pages9
JournalBiochemistry and Molecular Biology International
Volume31
Issue number6
StatePublished - 1993
Externally publishedYes

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