Modeling pulmonary hypertension: A pig model of postcapillary pulmonary hypertension

Olympia Bikou, Kiyotake Ishikawa, Kenneth M. Fish, Iratxe Zarragoikoetxea, Roger J. Hajjar, Jaume Aguero

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

7 Scopus citations

Abstract

Pulmonary hypertension (PH) is a pathophysiological condition defined as an increase in mean pulmonary arterial pressure ≥25, mmHg at rest assessed by right heart catheterization. Based on hemodynamic criteria, precapillary PH is characterized by a mean pulmonary capillary wedge pressure ≤15, mmHg as opposed to the postcapillary PH by >15, mmHg. Postcapillary PH is one of the most common forms of PH, often caused by left ventricular dysfunction and heart failure. In this chapter, we describe protocols for creating a large animal model of postcapillary PH. It is induced by open chest surgery (lateral thoracotomy) to band the pulmonary veins. The model is characterized by low mortality, relatively easy surgical procedure with well reproducible results, and pulmonary and cardiac remodeling at the structural, functional, and molecular levels. The presence of right ventricular (RV) remodeling is of significant importance since right heart failure is the main cause of death in patients suffering from PH. One of the advantages of the model described in this chapter is that both adaptive and maladaptive forms of RV remodeling can be observed during the progression of the disease. This can help understand the progressive pathophysiology of RV failure in humans. Besides the description of the model, a detailed guidance of the RV functional assessment in pigs for, both invasive (heart catheterization) and noninvasive (echocardiography) approaches, is provided.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages367-383
Number of pages17
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1816
ISSN (Print)1064-3745

Keywords

  • Animal model
  • Large animal
  • Postcapillary pulmonary hypertension
  • Pulmonary hypertension
  • Right heart echocardiography
  • Right heart failure

Fingerprint

Dive into the research topics of 'Modeling pulmonary hypertension: A pig model of postcapillary pulmonary hypertension'. Together they form a unique fingerprint.

Cite this