Modeling intratumor heterogeneity through CRISPR-barcodes

Alexis Guernet; Stuart A. Aaronson; Youssef Anouar; Luca Grumolato

doi:10.1080/23723556.2016.1227894

Modeling intratumor heterogeneity through CRISPR-barcodes

Alexis Guernet
, Stuart A. Aaronson
, Youssef Anouar
, Luca Grumolato

Research output: Contribution to journal › Comment/debate

7 Scopus citations

Abstract

We have devised a barcoding strategy to recapitulate cancer evolution through the emergence of subclonal mutations of interest, whose effects can be monitored in a dynamic manner. This approach can be easily adapted for a variety of applications, including combined modeling of multiple mechanisms of drug resistance or repair of oncogenic driver mutations in addicted cancer cells.

Original language	English
Article number	e1227894
Journal	Molecular and Cellular Oncology
Volume	3
Issue number	6
DOIs	https://doi.org/10.1080/23723556.2016.1227894
State	Published - 1 Nov 2016

Keywords

ALK
APC
CRISPR/Cas9
EGFR
TP53
genetic barcoding
non-small cell lung cancer
resistance
tumor heterogeneity

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10.1080/23723556.2016.1227894

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title = "Modeling intratumor heterogeneity through CRISPR-barcodes",

abstract = "We have devised a barcoding strategy to recapitulate cancer evolution through the emergence of subclonal mutations of interest, whose effects can be monitored in a dynamic manner. This approach can be easily adapted for a variety of applications, including combined modeling of multiple mechanisms of drug resistance or repair of oncogenic driver mutations in addicted cancer cells.",

keywords = "ALK, APC, CRISPR/Cas9, EGFR, TP53, genetic barcoding, non-small cell lung cancer, resistance, tumor heterogeneity",

author = "Alexis Guernet and Aaronson, \{Stuart A.\} and Youssef Anouar and Luca Grumolato",

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AU - Guernet, Alexis

AU - Aaronson, Stuart A.

AU - Anouar, Youssef

AU - Grumolato, Luca

PY - 2016/11/1

Y1 - 2016/11/1

N2 - We have devised a barcoding strategy to recapitulate cancer evolution through the emergence of subclonal mutations of interest, whose effects can be monitored in a dynamic manner. This approach can be easily adapted for a variety of applications, including combined modeling of multiple mechanisms of drug resistance or repair of oncogenic driver mutations in addicted cancer cells.

AB - We have devised a barcoding strategy to recapitulate cancer evolution through the emergence of subclonal mutations of interest, whose effects can be monitored in a dynamic manner. This approach can be easily adapted for a variety of applications, including combined modeling of multiple mechanisms of drug resistance or repair of oncogenic driver mutations in addicted cancer cells.

KW - ALK

KW - APC

KW - CRISPR/Cas9

KW - EGFR

KW - TP53

KW - genetic barcoding

KW - non-small cell lung cancer

KW - resistance

KW - tumor heterogeneity

UR - https://www.scopus.com/pages/publications/85027027636

U2 - 10.1080/23723556.2016.1227894

DO - 10.1080/23723556.2016.1227894

M3 - Comment/debate

AN - SCOPUS:85027027636

SN - 2372-3556

VL - 3

JO - Molecular and Cellular Oncology

JF - Molecular and Cellular Oncology

IS - 6

M1 - e1227894

ER -

Modeling intratumor heterogeneity through CRISPR-barcodes

Abstract

Keywords

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