MIWI-independent small RNAs (MSY-RNAs) bind to the RNA-binding protein, MSY2, in male germ cells

Mingang Xu; Sergey Medvedev; Juxiang Yang; Norman B. Hecht

doi:10.1073/pnas.0903944106

MIWI-independent small RNAs (MSY-RNAs) bind to the RNA-binding protein, MSY2, in male germ cells

Mingang Xu, Sergey Medvedev, Juxiang Yang, Norman B. Hecht

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

The germ cell-specific DNA/RNA-binding protein MSY2 binds small RNAs (MSY-RNAs) that are ≈25-31 nt in length, often initiate with a 5′ adenine, and are expressed in both germ cells and somatic cells. MSY-RNA levels do not decrease in Miwi or Msy2 null mice. Most MSY-RNAs map within annotated genes, but some are PIWI-interacting RNA (piRNA)-like and map to piRNA clusters. MSY-RNAs are in both nuclei and cytoplasm. In nuclei, MSY-RNAs are enriched in chromatin, and in the cytoplasm they are detected in both ribonucleoproteins and polysomes.

Original language	English
Pages (from-to)	12371-12376
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	106
Issue number	30
DOIs	https://doi.org/10.1073/pnas.0903944106
State	Published - 28 Jul 2009
Externally published	Yes

Keywords

Noncoding RNA
RNA-protein interactions
Spermatogenesis
Y box proteins

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10.1073/pnas.0903944106

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title = "MIWI-independent small RNAs (MSY-RNAs) bind to the RNA-binding protein, MSY2, in male germ cells",

abstract = "The germ cell-specific DNA/RNA-binding protein MSY2 binds small RNAs (MSY-RNAs) that are ≈25-31 nt in length, often initiate with a 5′ adenine, and are expressed in both germ cells and somatic cells. MSY-RNA levels do not decrease in Miwi or Msy2 null mice. Most MSY-RNAs map within annotated genes, but some are PIWI-interacting RNA (piRNA)-like and map to piRNA clusters. MSY-RNAs are in both nuclei and cytoplasm. In nuclei, MSY-RNAs are enriched in chromatin, and in the cytoplasm they are detected in both ribonucleoproteins and polysomes.",

keywords = "Noncoding RNA, RNA-protein interactions, Spermatogenesis, Y box proteins",

author = "Mingang Xu and Sergey Medvedev and Juxiang Yang and Hecht, \{Norman B.\}",

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doi = "10.1073/pnas.0903944106",

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T1 - MIWI-independent small RNAs (MSY-RNAs) bind to the RNA-binding protein, MSY2, in male germ cells

AU - Xu, Mingang

AU - Medvedev, Sergey

AU - Yang, Juxiang

AU - Hecht, Norman B.

PY - 2009/7/28

Y1 - 2009/7/28

N2 - The germ cell-specific DNA/RNA-binding protein MSY2 binds small RNAs (MSY-RNAs) that are ≈25-31 nt in length, often initiate with a 5′ adenine, and are expressed in both germ cells and somatic cells. MSY-RNA levels do not decrease in Miwi or Msy2 null mice. Most MSY-RNAs map within annotated genes, but some are PIWI-interacting RNA (piRNA)-like and map to piRNA clusters. MSY-RNAs are in both nuclei and cytoplasm. In nuclei, MSY-RNAs are enriched in chromatin, and in the cytoplasm they are detected in both ribonucleoproteins and polysomes.

AB - The germ cell-specific DNA/RNA-binding protein MSY2 binds small RNAs (MSY-RNAs) that are ≈25-31 nt in length, often initiate with a 5′ adenine, and are expressed in both germ cells and somatic cells. MSY-RNA levels do not decrease in Miwi or Msy2 null mice. Most MSY-RNAs map within annotated genes, but some are PIWI-interacting RNA (piRNA)-like and map to piRNA clusters. MSY-RNAs are in both nuclei and cytoplasm. In nuclei, MSY-RNAs are enriched in chromatin, and in the cytoplasm they are detected in both ribonucleoproteins and polysomes.

KW - Noncoding RNA

KW - RNA-protein interactions

KW - Spermatogenesis

KW - Y box proteins

UR - https://www.scopus.com/pages/publications/68149153185

U2 - 10.1073/pnas.0903944106

DO - 10.1073/pnas.0903944106

M3 - Article

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AN - SCOPUS:68149153185

SN - 0027-8424

VL - 106

SP - 12371

EP - 12376

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 30

ER -

MIWI-independent small RNAs (MSY-RNAs) bind to the RNA-binding protein, MSY2, in male germ cells

Abstract

Keywords

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