Mitochondrial Isolation and Real-Time Monitoring of MOMP

Mark P.A. Luna-Vargas, Jarvier N. Mohammed, Jesse D. Gelles, Jerry Edward Chipuk

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations


Isolated model systems have proven to be the standard in the apoptosis field to deconstruct MOMP into individual steps and to study the behavior of a subset of MOMP regulators. Here we describe the method to isolate, JC-1-label, and purify mouse liver mitochondria and subsequently describe how to utilize the JC-1-labeled mitochondria for real-time MOMP measurements.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages10
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • BAX
  • BCL-2 family
  • Isolated mitochondria
  • JC-1 labeling
  • Real-time MOMP measurements


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