MiRNome Profiling of Extracellular Vesicles in Patients with Severe COVID-19 and Identification of Predictors of Mortality

Laura Sánchez-De Prada, Adrián García-Concejo, Álvaro Tamayo-Velasco, Marta Martín-Fernández, Hugo Gonzalo-Benito, Óscar Gorgojo-Galindo, A. Montero-Jodra, María Teresa Peláez, Iciar Martínez Almeida, Miguel Bardají-Carrillo, Rocío López-Herrero, Patricia Román-García, José María Eiros, Iván Sanz-Muñoz, Teresa Aydillo, María Ángeles Jiménez-Sousa, Amanda Fernández-Rodríguez, Salvador Resino, María Heredia-Rodríguez, David BernardoEster Gómez-Sánchez, Eduardo Tamayo

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Background: Extracellular vesicles (EVs), containing microRNAs (miRNAs) and other molecules, play a central role in intercellular communication, especially in viral infections caused by SARS-CoV-2. This study explores the miRNA profiles in plasma-derived EVs from patients with severe COVID-19 vs controls, identifying potential mortality predictors. Methods: This prospective study included 36 patients with severe COVID-19 and 33 controls without COVID-19. EV-derived miRNAs were sequenced, and bioinformatics and differential expression analysis between groups were performed. The plasma miRNA profile of an additional cohort of patients with severe COVID-19 (n = 32) and controls (n = 12) was used to compare with our data. Survival analysis identified potential mortality predictors among the significantly differentially expressed (SDE) miRNAs in EVs. Results: Patients with severe COVID-19 showed 50 SDE miRNAs in plasma-derived EVs. These miRNAs were associated with pathways related to inflammation and cell adhesion. Fifteen of these plasma-derived EV miRNAs were SDE in the plasma of severe cases vs controls. Two miRNAs, hsa-miR-1469 and hsa-miR-6124, were identified as strong mortality predictors with an area under the receiver operating characteristic curve of 0.938. Conclusions: This research provides insights into the role of miRNAs within EVs in severe COVID-19 and their potential as clinical biomarkers for mortality.

Original languageEnglish
Pages (from-to)901-911
Number of pages11
JournalJournal of Infectious Diseases
Volume230
Issue number4
DOIs
StatePublished - 15 Oct 2024

Keywords

  • COVID-19
  • critically ill patients
  • extracellular vesicles
  • microRNAs
  • mortality biomarkers

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