TY - JOUR
T1 - MiRNome Profiling of Extracellular Vesicles in Patients with Severe COVID-19 and Identification of Predictors of Mortality
AU - Sánchez-De Prada, Laura
AU - García-Concejo, Adrián
AU - Tamayo-Velasco, Álvaro
AU - Martín-Fernández, Marta
AU - Gonzalo-Benito, Hugo
AU - Gorgojo-Galindo, Óscar
AU - Montero-Jodra, A.
AU - Peláez, María Teresa
AU - Martínez Almeida, Iciar
AU - Bardají-Carrillo, Miguel
AU - López-Herrero, Rocío
AU - Román-García, Patricia
AU - Eiros, José María
AU - Sanz-Muñoz, Iván
AU - Aydillo, Teresa
AU - Jiménez-Sousa, María Ángeles
AU - Fernández-Rodríguez, Amanda
AU - Resino, Salvador
AU - Heredia-Rodríguez, María
AU - Bernardo, David
AU - Gómez-Sánchez, Ester
AU - Tamayo, Eduardo
N1 - Publisher Copyright:
© 2024 The Author(s). Published by Oxford University Press on behalf of Infectious Diseases Society of America. All rights reserved.
PY - 2024/10/15
Y1 - 2024/10/15
N2 - Background: Extracellular vesicles (EVs), containing microRNAs (miRNAs) and other molecules, play a central role in intercellular communication, especially in viral infections caused by SARS-CoV-2. This study explores the miRNA profiles in plasma-derived EVs from patients with severe COVID-19 vs controls, identifying potential mortality predictors. Methods: This prospective study included 36 patients with severe COVID-19 and 33 controls without COVID-19. EV-derived miRNAs were sequenced, and bioinformatics and differential expression analysis between groups were performed. The plasma miRNA profile of an additional cohort of patients with severe COVID-19 (n = 32) and controls (n = 12) was used to compare with our data. Survival analysis identified potential mortality predictors among the significantly differentially expressed (SDE) miRNAs in EVs. Results: Patients with severe COVID-19 showed 50 SDE miRNAs in plasma-derived EVs. These miRNAs were associated with pathways related to inflammation and cell adhesion. Fifteen of these plasma-derived EV miRNAs were SDE in the plasma of severe cases vs controls. Two miRNAs, hsa-miR-1469 and hsa-miR-6124, were identified as strong mortality predictors with an area under the receiver operating characteristic curve of 0.938. Conclusions: This research provides insights into the role of miRNAs within EVs in severe COVID-19 and their potential as clinical biomarkers for mortality.
AB - Background: Extracellular vesicles (EVs), containing microRNAs (miRNAs) and other molecules, play a central role in intercellular communication, especially in viral infections caused by SARS-CoV-2. This study explores the miRNA profiles in plasma-derived EVs from patients with severe COVID-19 vs controls, identifying potential mortality predictors. Methods: This prospective study included 36 patients with severe COVID-19 and 33 controls without COVID-19. EV-derived miRNAs were sequenced, and bioinformatics and differential expression analysis between groups were performed. The plasma miRNA profile of an additional cohort of patients with severe COVID-19 (n = 32) and controls (n = 12) was used to compare with our data. Survival analysis identified potential mortality predictors among the significantly differentially expressed (SDE) miRNAs in EVs. Results: Patients with severe COVID-19 showed 50 SDE miRNAs in plasma-derived EVs. These miRNAs were associated with pathways related to inflammation and cell adhesion. Fifteen of these plasma-derived EV miRNAs were SDE in the plasma of severe cases vs controls. Two miRNAs, hsa-miR-1469 and hsa-miR-6124, were identified as strong mortality predictors with an area under the receiver operating characteristic curve of 0.938. Conclusions: This research provides insights into the role of miRNAs within EVs in severe COVID-19 and their potential as clinical biomarkers for mortality.
KW - COVID-19
KW - critically ill patients
KW - extracellular vesicles
KW - microRNAs
KW - mortality biomarkers
UR - http://www.scopus.com/inward/record.url?scp=85202620218&partnerID=8YFLogxK
U2 - 10.1093/infdis/jiae310
DO - 10.1093/infdis/jiae310
M3 - Article
C2 - 38865487
AN - SCOPUS:85202620218
SN - 0022-1899
VL - 230
SP - 901
EP - 911
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 4
ER -