Abstract
We have investigated the minimal requirements for a functional origin of DNA replication in an in vitro assay which requires the adeno-associated virus (AAV) Rep 68/78 protein. When a linear duplex template was used, initiation of one round of DNA replication was achieved when nucleotides 1 ˜56 of the AAV inverted terminal repeat (in the flop orientation) were present at the terminus of the template. A terminal resolution site was not required. Deletion of an additional 11 nucleotides from the terminal repeat blocked the reaction.
| Original language | English |
|---|---|
| Article number | 71306 |
| Pages (from-to) | 692-695 |
| Number of pages | 4 |
| Journal | Virology |
| Volume | 209 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1 Jun 1995 |
| Externally published | Yes |