TY - JOUR
T1 - Migration and differentiation of neural precursors derived from human embryonic stem cells in the rat brain
AU - Tabar, Viviane
AU - Panagiotakos, Georgia
AU - Greenberg, Edward D.
AU - Chan, Bill K.
AU - Sadelain, Michel
AU - Gutin, Philip H.
AU - Studer, Lorenz
PY - 2005
Y1 - 2005
N2 - Human embryonic stem (hES) cells provide a potentially unlimited cell source for regenerative medicine. Recently, differentiation strategies were developed to direct hES cells towards neural fates in vitro. However, the interaction of hES cell progeny with the adult brain environment remains unexplored. Here we report that hES cell-derived neural precursors differentiate into neurons, astrocytes and oligodendrocytes in the normal and lesioned brain of young adult rats and migrate extensively along white matter tracts. The differentiation and migration behavior of hES cell progeny was region specific. The hES cell-derived neural precursors integrated into the endogenous precursor pool in the subventricular zone, a site of persistent neurogenesis. Like adult neural stem cells, hES cell-derived precursors traveled along the rostral migratory stream to the olfactory bulb, where they contributed to neurogenesis. We found no evidence of cell fusion, suggesting that hES cell progeny are capable of responding appropriately to host cues in the subventricular zone.
AB - Human embryonic stem (hES) cells provide a potentially unlimited cell source for regenerative medicine. Recently, differentiation strategies were developed to direct hES cells towards neural fates in vitro. However, the interaction of hES cell progeny with the adult brain environment remains unexplored. Here we report that hES cell-derived neural precursors differentiate into neurons, astrocytes and oligodendrocytes in the normal and lesioned brain of young adult rats and migrate extensively along white matter tracts. The differentiation and migration behavior of hES cell progeny was region specific. The hES cell-derived neural precursors integrated into the endogenous precursor pool in the subventricular zone, a site of persistent neurogenesis. Like adult neural stem cells, hES cell-derived precursors traveled along the rostral migratory stream to the olfactory bulb, where they contributed to neurogenesis. We found no evidence of cell fusion, suggesting that hES cell progeny are capable of responding appropriately to host cues in the subventricular zone.
UR - http://www.scopus.com/inward/record.url?scp=22444449591&partnerID=8YFLogxK
U2 - 10.1038/nbt1088
DO - 10.1038/nbt1088
M3 - Article
C2 - 15852001
AN - SCOPUS:22444449591
SN - 1087-0156
VL - 23
SP - 601
EP - 606
JO - Nature Biotechnology
JF - Nature Biotechnology
IS - 5
ER -