Migration and differentiation of human umbilical cord mononuclear cells in the brain of neonate rats following hypoxic-ischemic brain damage

Xiao Li Wang, Yan Song Zhao, Yao Wu Li, Min Xu

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND: Umbilical cord blood mononuclear cell (UMNC) transplantation in the brain can survive, reduce brain damage induced by hypoxia and ischemia, significantly improve long-term ethology in rats following brain damage, but the mechanisms are still unclear. OBJECTIVE: To investigate the migration and differentiation of human umbilical cord mononuclear cells after their transplantation to the brain via lateral ventricle in rats with brain damage induced by hypoxia and ischemia. DESIGN, TIME AND SETTING: The in vivo cytological study was performed at the Molecular Imaging Center, Weifang Medical College from December 2007 to August 2008. MATERIALS: Umbilical cord blood was obtained from healthy full-term pregnant women at the Department of Obstetrics, Hospital Affiliated to Weifang Medical College. A total of 50 clean healthy Sprague Dawley neonatal rats aged 7 days were supplied by Animal Center, Shandong University of Traditional Chinese Medicine. METHODS: UMNCs were obtained from human cord blood in vitro by Ficoll method, and labeled by BrdU 3 days prior to transplantation. A total of 50 rat models of hypoxic-ischemic brain damage were established by Rice-Vannucci method. UMNC suspension was infused into the left lateral ventricle (AP: -0.5 mm, ML: -2 mm, DV: -2 mm) 24 hours following model establishment, 2 μL/point, totally 1×10 5 living cells. Brain tissue sections were prepared 24 hours, 3, 7, 14 and 28 days following transplantation, 10 rats per time point. MAIN OUTCOME MEASURES: Migration of UMNCs, expression and differentiation of neural stem cells in the brain were measured using double immunofluorescent staining. RESULTS: All the 50 rats were involved in final analysis. Circled digit one24 hours after transplantation, BrdU+ cells were seen in the lateral ventricular; 3 and 7 days after transplantation, the BrdU+ cells were observed in the subventricular zone; 14 and 28 days after transplantation, the BrdU+ cells migrated to the cortex and hippocampus. Circled digit two24 hours after transplantation, BrdU+nestin+ cells were observed in the lateral ventricle zone, and 3 days after transplantation, in the subependymal region. 7 d after transplantation, the number of BrdU +nestin+ cells was increased and decreased 14 d after transplantation. Circled digit threeBrdU+NSE+ cells and BrdU+GFAP+ cells were observed in the cortex and hippocampus 14 days after transplantation. Both the number of double positive cells and the ratios of BrdU+NSE+ cells and BrdU +GFAP+ cells to BrdU+ cells were increased 28 days after transplantation. CONCLUSION: UMNCs can survive, migrate to the cortex and hippocampus and then differentiate into mature neurons and astrocytes after transplantation.

Original languageEnglish
Pages (from-to)6259-6262
Number of pages4
JournalChinese Journal of Tissue Engineering Research
Volume13
Issue number32
DOIs
StatePublished - 6 Aug 2009
Externally publishedYes

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