TY - JOUR
T1 - MicroRNA editing in seed region aligns with cellular changes in hypoxic conditions
AU - Nigita, Giovanni
AU - Acunzo, Mario
AU - Romano, Giulia
AU - Veneziano, Dario
AU - Laganà, Alessandro
AU - Vitiello, Marika
AU - Wernicke, Dorothee
AU - Ferro, Alfredo
AU - Croce, Carlo M.
N1 - Publisher Copyright:
© 2016 The Author(s). Published by Oxford University Press on behalf of Nucleic Acids Research.
PY - 2016/7/27
Y1 - 2016/7/27
N2 - RNA editing is a finely tuned, dynamic mechanism for post-transcriptional gene regulation that has been thoroughly investigated in the last decade. Nevertheless, RNA editing in non-coding RNA, such as microRNA (miRNA), have caused great debate and have called for deeper investigation. Until recently, in fact, inadequate methodologies and experimental contexts have been unable to provide detailed insights for further elucidation of RNA editing affecting miRNAs, especially in cancer. In this work, we leverage on recent innovative bioinformatics approaches applied to a more informative experimental context in order to analyze the variations in miRNA seed region editing activity during a time course of a hypoxia-exposed breast cancer cell line. By investigating its behavior in a dynamic context, we found that miRNA editing events in the seed region are not depended on miRNA expression, unprecedentedly providing insights on the targetome shifts derived from these modifications. This reveals that miRNA editing acts under the influence of environmentally induced stimuli. Our results show a miRNA editing activity trend aligning with cellular pathways closely associated to hypoxia, such as the VEGF and PI3K/Akt pathways, providing important novel insights on this poorly elucidated phenomenon.
AB - RNA editing is a finely tuned, dynamic mechanism for post-transcriptional gene regulation that has been thoroughly investigated in the last decade. Nevertheless, RNA editing in non-coding RNA, such as microRNA (miRNA), have caused great debate and have called for deeper investigation. Until recently, in fact, inadequate methodologies and experimental contexts have been unable to provide detailed insights for further elucidation of RNA editing affecting miRNAs, especially in cancer. In this work, we leverage on recent innovative bioinformatics approaches applied to a more informative experimental context in order to analyze the variations in miRNA seed region editing activity during a time course of a hypoxia-exposed breast cancer cell line. By investigating its behavior in a dynamic context, we found that miRNA editing events in the seed region are not depended on miRNA expression, unprecedentedly providing insights on the targetome shifts derived from these modifications. This reveals that miRNA editing acts under the influence of environmentally induced stimuli. Our results show a miRNA editing activity trend aligning with cellular pathways closely associated to hypoxia, such as the VEGF and PI3K/Akt pathways, providing important novel insights on this poorly elucidated phenomenon.
UR - http://www.scopus.com/inward/record.url?scp=84982805924&partnerID=8YFLogxK
U2 - 10.1093/nar/gkw532
DO - 10.1093/nar/gkw532
M3 - Article
C2 - 27298257
AN - SCOPUS:84982805924
SN - 0305-1048
VL - 44
SP - 6298
EP - 6308
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 13
ER -