Microparticles released by Listeria monocytogenes-infected macrophages are required for dendritic cell-elicited protective immunity

Yi Zhang, Ruihua Zhang, Huafeng Zhang, Jing Liu, Zhuoshun Yang, Pingwei Xu, Wenqian Cai, Geming Lu, Miao Cui, Reto A. Schwendener, Huang Zhong Shi, Huabao Xiong, Bo Huang

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Interplay between macrophages and dendritic cells in the processing and presentation of bacterial antigens for T-cell immune responses remains poorly understood. Using a Listeria monocytogenes (Lm) infection model, we demonstrate that dendritic cells (DCs) require the support of macrophages to elicit protective immunity against Lm infection. DCs themselves were inefficient at taking up Lm but capable of taking up microparticles (MPs) released by Lm-infected macrophages. These MPs transferred Lm antigens to DCs, allowing DCs to present Lm antigen to effector T cells. MP-mediated Lm antigen transfer required MHC class I participation, since MHC class I deficiency in macrophages resulted in a significant reduction of T-cell activation. Moreover, the vaccination of mice with MPs from Lm-infected macrophages produced strong protective immunity against Lm infection. We here identify an intrinsic antigen transfer program between macrophages and DCs during Lm infection, and emphasize that macrophages also play an essential role in DC-elicited Lm-specific T-cell responses.

Original languageEnglish
Pages (from-to)489-496
Number of pages8
JournalCellular and Molecular Immunology
Volume9
Issue number6
DOIs
StatePublished - Nov 2012

Keywords

  • Dendritic cell
  • Listeria monocytogenes
  • Macrophage
  • Microparticles
  • Protective immunity

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