Microfluidic electroporation for delivery of cell-penetrating peptide conjugates of peptide nucleic acids (PNA) for antisense inhibition of intracellular bacteria

Sai Ma, Betsy Schroeder, Chen Sun, Despina Nelie Loufakis, Zhenning Cao, Nammalwar Sriranganathan, Chang Lu

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Cell penetrating peptides (CPPs) have been used for a myriad of cellular delivery applications and were recently explored for delivery of antisense agents such as peptide nucleic acids (PNAs) for bacterial inhibition. In this report, we show that microfluidic electroporation delivery dramatically increases the bioavailability of CPP-PNAs to kill Salmonella macrophages which is typical in animals and humans. Electroporation delivers the molecules without involving endocytosis and greatly increases the antisense effect. The decrease in the average number of Salmonella per macrophage under a 1200 V/cm and 5 ms pulse was a factor of 9 higher than that without electroporation.

Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages962-964
Number of pages3
ISBN (Electronic)9780979806476
StatePublished - 2014
Externally publishedYes
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: 26 Oct 201430 Oct 2014

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Conference

Conference18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Country/TerritoryUnited States
CitySan Antonio
Period26/10/1430/10/14

Keywords

  • Cell penetrating peptide
  • Electroporation
  • Peptide nucleic acids

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