Microchemical assay of galactose containing cerebrosides

H. S. Maker; G. M. Lehrer; C. Weiss; L. Levine

doi:10.1016/0003-2697(74)90414-X

Microchemical assay of galactose containing cerebrosides

H. S. Maker, G. M. Lehrer, C. Weiss, L. Levine

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Abstract

A method for the estimation of galactose containing lipids based on acid hydrolysis and fluorometric assay of galactose with galactose dehydrogenase has been developed. The characteristics of cerebroside hydrolysis under several different conditions are described. Under the conditions of hydrolysis sphingosine and galactose are released in parallel. A microchemical modification of the procedure has been used to assay galactose cerebroside-sulfatide in fragments of mouse cerebellar layers weighing 1-3 μg dry wt dissected from freeze-dried tissue sections.

Original language	English
Pages (from-to)	471-478
Number of pages	8
Journal	Analytical Biochemistry
Volume	61
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(74)90414-X
State	Published - Oct 1974
Externally published	Yes

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title = "Microchemical assay of galactose containing cerebrosides",

abstract = "A method for the estimation of galactose containing lipids based on acid hydrolysis and fluorometric assay of galactose with galactose dehydrogenase has been developed. The characteristics of cerebroside hydrolysis under several different conditions are described. Under the conditions of hydrolysis sphingosine and galactose are released in parallel. A microchemical modification of the procedure has been used to assay galactose cerebroside-sulfatide in fragments of mouse cerebellar layers weighing 1-3 μg dry wt dissected from freeze-dried tissue sections.",

author = "Maker, {H. S.} and Lehrer, {G. M.} and C. Weiss and L. Levine",

note = "Funding Information: This study was supported by National Institute of Neurological Diseases and Stroke Grant No. NS 06224, National Multiple Sclerosis Society Grant No. 722, John A. Hartford Foundation and from the National Institute of Mental Health Grant No. MH 20786.",

year = "1974",

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doi = "10.1016/0003-2697(74)90414-X",

language = "English",

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journal = "Analytical Biochemistry",

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T1 - Microchemical assay of galactose containing cerebrosides

AU - Maker, H. S.

AU - Lehrer, G. M.

AU - Weiss, C.

AU - Levine, L.

N1 - Funding Information: This study was supported by National Institute of Neurological Diseases and Stroke Grant No. NS 06224, National Multiple Sclerosis Society Grant No. 722, John A. Hartford Foundation and from the National Institute of Mental Health Grant No. MH 20786.

PY - 1974/10

Y1 - 1974/10

N2 - A method for the estimation of galactose containing lipids based on acid hydrolysis and fluorometric assay of galactose with galactose dehydrogenase has been developed. The characteristics of cerebroside hydrolysis under several different conditions are described. Under the conditions of hydrolysis sphingosine and galactose are released in parallel. A microchemical modification of the procedure has been used to assay galactose cerebroside-sulfatide in fragments of mouse cerebellar layers weighing 1-3 μg dry wt dissected from freeze-dried tissue sections.

AB - A method for the estimation of galactose containing lipids based on acid hydrolysis and fluorometric assay of galactose with galactose dehydrogenase has been developed. The characteristics of cerebroside hydrolysis under several different conditions are described. Under the conditions of hydrolysis sphingosine and galactose are released in parallel. A microchemical modification of the procedure has been used to assay galactose cerebroside-sulfatide in fragments of mouse cerebellar layers weighing 1-3 μg dry wt dissected from freeze-dried tissue sections.

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U2 - 10.1016/0003-2697(74)90414-X

DO - 10.1016/0003-2697(74)90414-X

M3 - Article

C2 - 4422683

AN - SCOPUS:0016304757

SN - 0003-2697

VL - 61

SP - 471

EP - 478

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Microchemical assay of galactose containing cerebrosides

Abstract

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