TY - JOUR
T1 - Microarray applications in neuroscience
AU - Luo, Z.
AU - Geschwind, D. H.
N1 - Funding Information:
We thank Ms. Bonita Porch for her editorial assistance and Ms. Carol Gray of the Media Core of the UCLA Mental Retardation Research Center for her help with the figure. We acknowledge the National Institute of Mental Health (Grant MH60233) and the James F. Williams Neuroscience Fund for support of our microarray research. We also thank Robert Collins, M.D. (Chair, UCLA Department of Neurology) for his support of the Neurogenetics Program.
PY - 2001
Y1 - 2001
N2 - Advances in all facets of technology from molecular biology to imaging and computational biology offer unprecedented opportunities for improving our understanding of the brain in health and disease. Oligonucleotide and cDNA microarray analysis, using a variety of "DNA chips," is a recently developed high-throughput technique that allows for tour-de-force analysis of gene expression. We review this powerful technique, developed in genetics laboratories, with reference to applications in neurologic diseases in humans and the use of animal models. The typical microarray experiment is multistaged and includes preparation or purchase of arrays, preparation of target DNA and probe, target DNA hybridization, microarray scanning, and image analysis. The power and pitfalls of this technology are discussed in the context of neuroscience paradigms. Since unprecedented amounts of data are produced from microarray experiments, bioinformatics and modeling expertise are increasingly becoming critical components of this approach.
AB - Advances in all facets of technology from molecular biology to imaging and computational biology offer unprecedented opportunities for improving our understanding of the brain in health and disease. Oligonucleotide and cDNA microarray analysis, using a variety of "DNA chips," is a recently developed high-throughput technique that allows for tour-de-force analysis of gene expression. We review this powerful technique, developed in genetics laboratories, with reference to applications in neurologic diseases in humans and the use of animal models. The typical microarray experiment is multistaged and includes preparation or purchase of arrays, preparation of target DNA and probe, target DNA hybridization, microarray scanning, and image analysis. The power and pitfalls of this technology are discussed in the context of neuroscience paradigms. Since unprecedented amounts of data are produced from microarray experiments, bioinformatics and modeling expertise are increasingly becoming critical components of this approach.
UR - http://www.scopus.com/inward/record.url?scp=0035029243&partnerID=8YFLogxK
U2 - 10.1006/nbdi.2001.0387
DO - 10.1006/nbdi.2001.0387
M3 - Review article
C2 - 11300716
AN - SCOPUS:0035029243
SN - 0969-9961
VL - 8
SP - 183
EP - 193
JO - Neurobiology of Disease
JF - Neurobiology of Disease
IS - 2
ER -