@inbook{c6f15c8a526d4e029225789361375ab8,
title = "Methods for Studying Checkpoint Kinases – Chk1",
abstract = "Attempts to passage through mitosis with unrepaired DNA damage or incompletely replicated DNA leads to genome instability and/or cell death. To prevent this from occurring, an ancient checkpoint (known as the G2 DNA damage checkpoint) that inhibits the activation of the mitotic cyclin-dependent kinase is activated to hold cells in the G2 phase of the cell cycle. The effector of this checkpoint is Chk1, a protein serine-threonine kinase. Chk1 contains an N-terminal catalytic domain, and C-terminal regulatory domain. Within the regulatory domain there are two residues, Serine-317 (S317) and Serine-345 (S345), which are phosphorylated in active Chk1 molecules, and subsequently dephosphorylated to inactivate Chk1 and allow mitotic entry. Phospho-specific antibodies can be used to detect these activating phosphorylations, and this provides a simple and sensitive marker of Chk1 activation.",
keywords = "Chk1, DNA damage, Phosphorylation, Western blotting, checkpoint",
author = "Claudia Tapia-Alveal and O{\textquoteright}Connell, {Matthew J.}",
note = "Publisher Copyright: {\textcopyright} 2011, Springer Science+Business Media, LLC.",
year = "2011",
doi = "10.1007/978-1-61779-273-1_12",
language = "English",
isbn = "9781617792724",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "171--179",
booktitle = "Cell Cycle Checkpoints",
}