Methodological guidelines to study extracellular vesicles

Frank A.W. Coumans; Alain R. Brisson; Edit I. Buzas; Françoise Dignat-George; Esther E.E. Drees; Samir El-Andaloussi; Costanza Emanueli; Aleksandra Gasecka; An Hendrix; Andrew F. Hill; Romaric Lacroix; Yi Lee; Ton G. Van Leeuwen; Nigel Mackman; Imre Mäger; John P. Nolan; Edwin Van Der Pol; D. Michiel Pegtel; Susmita Sahoo; Pia R.M. Siljander; Guus Sturk; Olivier De Wever; Rienk Nieuwland

doi:10.1161/CIRCRESAHA.117.309417

Methodological guidelines to study extracellular vesicles

Frank A.W. Coumans, Alain R. Brisson, Edit I. Buzas, Françoise Dignat-George, Esther E.E. Drees, Samir El-Andaloussi, Costanza Emanueli, Aleksandra Gasecka, An Hendrix, Andrew F. Hill, Romaric Lacroix, Yi Lee, Ton G. Van Leeuwen, Nigel Mackman, Imre Mäger, John P. Nolan, Edwin Van Der Pol, D. Michiel Pegtel, Susmita Sahoo, Pia R.M. SiljanderGuus Sturk, Olivier De Wever, Rienk Nieuwland

Research output: Contribution to journal › Review article › peer-review

733 Scopus citations

Abstract

Owing to the relationship between extracellular vesicles (EVs) and physiological and pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes for novel diagnostic and translational discoveries. This review provides an expert-based update of recent advances in the methods to study EVs and summarizes currently accepted considerations and recommendations from sample collection to isolation, detection, and characterization of EVs. Common misconceptions and methodological pitfalls are highlighted. Although EVs are found in all body fluids, in this review, we will focus on EVs from human blood, not only our most complex but also the most interesting body fluid for cardiovascular research.

Original language	English
Pages (from-to)	1632-1648
Number of pages	17
Journal	Circulation Research
Volume	120
Issue number	10
DOIs	https://doi.org/10.1161/CIRCRESAHA.117.309417
State	Published - 12 May 2017

Keywords

cardiovascular diseases
exosomes
extracellular vesicles
methods
reference standards

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10.1161/CIRCRESAHA.117.309417

Cite this

Coumans, F. A. W., Brisson, A. R., Buzas, E. I., Dignat-George, F., Drees, E. E. E., El-Andaloussi, S., Emanueli, C., Gasecka, A., Hendrix, A., Hill, A. F., Lacroix, R., Lee, Y., Van Leeuwen, T. G., Mackman, N., Mäger, I., Nolan, J. P., Van Der Pol, E., Pegtel, D. M., Sahoo, S., ... Nieuwland, R. (2017). Methodological guidelines to study extracellular vesicles. Circulation Research, 120(10), 1632-1648. https://doi.org/10.1161/CIRCRESAHA.117.309417

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abstract = "Owing to the relationship between extracellular vesicles (EVs) and physiological and pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes for novel diagnostic and translational discoveries. This review provides an expert-based update of recent advances in the methods to study EVs and summarizes currently accepted considerations and recommendations from sample collection to isolation, detection, and characterization of EVs. Common misconceptions and methodological pitfalls are highlighted. Although EVs are found in all body fluids, in this review, we will focus on EVs from human blood, not only our most complex but also the most interesting body fluid for cardiovascular research.",

keywords = "cardiovascular diseases, exosomes, extracellular vesicles, methods, reference standards",

author = "Coumans, {Frank A.W.} and Brisson, {Alain R.} and Buzas, {Edit I.} and Fran{\c c}oise Dignat-George and Drees, {Esther E.E.} and Samir El-Andaloussi and Costanza Emanueli and Aleksandra Gasecka and An Hendrix and Hill, {Andrew F.} and Romaric Lacroix and Yi Lee and {Van Leeuwen}, {Ton G.} and Nigel Mackman and Imre M{\"a}ger and Nolan, {John P.} and {Van Der Pol}, Edwin and Pegtel, {D. Michiel} and Susmita Sahoo and Siljander, {Pia R.M.} and Guus Sturk and {De Wever}, Olivier and Rienk Nieuwland",

note = "Publisher Copyright: {\textcopyright} 2017 American Heart Association, Inc.",

year = "2017",

month = may,

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doi = "10.1161/CIRCRESAHA.117.309417",

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Coumans, FAW, Brisson, AR, Buzas, EI, Dignat-George, F, Drees, EEE, El-Andaloussi, S, Emanueli, C, Gasecka, A, Hendrix, A, Hill, AF, Lacroix, R, Lee, Y, Van Leeuwen, TG, Mackman, N, Mäger, I, Nolan, JP, Van Der Pol, E, Pegtel, DM, Sahoo, S, Siljander, PRM, Sturk, G, De Wever, O & Nieuwland, R 2017, 'Methodological guidelines to study extracellular vesicles', Circulation Research, vol. 120, no. 10, pp. 1632-1648. https://doi.org/10.1161/CIRCRESAHA.117.309417

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AU - Coumans, Frank A.W.

AU - Brisson, Alain R.

AU - Buzas, Edit I.

AU - Dignat-George, Françoise

AU - Drees, Esther E.E.

AU - El-Andaloussi, Samir

AU - Emanueli, Costanza

AU - Gasecka, Aleksandra

AU - Hendrix, An

AU - Hill, Andrew F.

AU - Lacroix, Romaric

AU - Lee, Yi

AU - Van Leeuwen, Ton G.

AU - Mackman, Nigel

AU - Mäger, Imre

AU - Nolan, John P.

AU - Van Der Pol, Edwin

AU - Pegtel, D. Michiel

AU - Sahoo, Susmita

AU - Siljander, Pia R.M.

AU - Sturk, Guus

AU - De Wever, Olivier

AU - Nieuwland, Rienk

PY - 2017/5/12

Y1 - 2017/5/12

N2 - Owing to the relationship between extracellular vesicles (EVs) and physiological and pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes for novel diagnostic and translational discoveries. This review provides an expert-based update of recent advances in the methods to study EVs and summarizes currently accepted considerations and recommendations from sample collection to isolation, detection, and characterization of EVs. Common misconceptions and methodological pitfalls are highlighted. Although EVs are found in all body fluids, in this review, we will focus on EVs from human blood, not only our most complex but also the most interesting body fluid for cardiovascular research.

AB - Owing to the relationship between extracellular vesicles (EVs) and physiological and pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes for novel diagnostic and translational discoveries. This review provides an expert-based update of recent advances in the methods to study EVs and summarizes currently accepted considerations and recommendations from sample collection to isolation, detection, and characterization of EVs. Common misconceptions and methodological pitfalls are highlighted. Although EVs are found in all body fluids, in this review, we will focus on EVs from human blood, not only our most complex but also the most interesting body fluid for cardiovascular research.

KW - cardiovascular diseases

KW - exosomes

KW - extracellular vesicles

KW - methods

KW - reference standards

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VL - 120

SP - 1632

EP - 1648

JO - Circulation Research

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IS - 10

ER -

Methodological guidelines to study extracellular vesicles

Abstract

Keywords

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