TY - JOUR
T1 - Methodological aspects of the molecular and histological study of prostate cancer
T2 - Focus on PTEN
AU - Ugalde-Olano, Aitziber
AU - Egia, Ainara
AU - Fernández-Ruiz, Sonia
AU - Loizaga-Iriarte, Ana
AU - Zuñiga-García, Patricia
AU - Garcia, Stephane
AU - Royo, Félix
AU - Lacasa-Viscasillas, Isabel
AU - Castro, Erika
AU - Cortazar, Ana R.
AU - Zabala-Letona, Amaia
AU - Martín-Martín, Natalia
AU - Arruabarrena-Aristorena, Amaia
AU - Torrano-Moya, Verónica
AU - Valcárcel-Jiménez, Lorea
AU - Sánchez-Mosquera, Pilar
AU - Caro-Maldonado, Alfredo
AU - González-Tampan, Jorge
AU - Cachi-Fuentes, Guido
AU - Bilbao, Elena
AU - Montero, Rocío
AU - Fernández, Sara
AU - Arrieta, Edurne
AU - Zorroza, Kerman
AU - Castillo-Martín, Mireia
AU - Serra, Violeta
AU - Salazar, Eider
AU - Macías-Cámara, Nuria
AU - Tabernero, Jose
AU - Baselga, Jose
AU - Cordón-Cardo, Carlos
AU - Aransay, Ana M.
AU - Villar, Amaia Del
AU - Iovanna, Juan L.
AU - Falcón-Pérez, Juan M.
AU - Unda, Miguel
AU - Bilbao, Roberto
AU - Carracedo, Arkaitz
N1 - Publisher Copyright:
© 2015.
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Prostate cancer is among the most frequent cancers in men, and despite its high rate of cure, the high number of cases results in an elevated mortality worldwide. Importantly, prostate cancer incidence is dramatically increasing in western societies in the past decades, suggesting that this type of tumor is exquisitely sensitive to lifestyle changes. Prostate cancer frequently exhibits alterations in the PTEN gene (inactivating mutations or gene deletions) or at the protein level (reduced protein expression or altered sub-cellular compartmentalization). The relevance of PTEN in this type of cancer is further supported by the fact that the sole deletion of PTEN in the murine prostate epithelium recapitulates many of the features of the human disease. In order to study the molecular alterations in prostate cancer, we need to overcome the methodological challenges that this tissue imposes. In this review we present protocols and methods, using PTEN as proof of concept, to study different molecular characteristics of prostate cancer.
AB - Prostate cancer is among the most frequent cancers in men, and despite its high rate of cure, the high number of cases results in an elevated mortality worldwide. Importantly, prostate cancer incidence is dramatically increasing in western societies in the past decades, suggesting that this type of tumor is exquisitely sensitive to lifestyle changes. Prostate cancer frequently exhibits alterations in the PTEN gene (inactivating mutations or gene deletions) or at the protein level (reduced protein expression or altered sub-cellular compartmentalization). The relevance of PTEN in this type of cancer is further supported by the fact that the sole deletion of PTEN in the murine prostate epithelium recapitulates many of the features of the human disease. In order to study the molecular alterations in prostate cancer, we need to overcome the methodological challenges that this tissue imposes. In this review we present protocols and methods, using PTEN as proof of concept, to study different molecular characteristics of prostate cancer.
KW - Fresh tissue
KW - Molecular biology
KW - PTEN
KW - Prostate cancer
UR - https://www.scopus.com/pages/publications/84959852057
U2 - 10.1016/j.ymeth.2015.02.005
DO - 10.1016/j.ymeth.2015.02.005
M3 - Article
C2 - 25697760
AN - SCOPUS:84959852057
SN - 1046-2023
VL - 77
SP - 25
EP - 30
JO - Methods
JF - Methods
ER -