@inbook{d3d0d7b50fa54c9cbff4d1754fa12154,
title = "Method Protocols for Metabolic and Functional Analysis of the BRIN-BD11 β-Cell Line: A Preclinical Model for Type 2 Diabetes",
abstract = "In type 2 diabetes, prolonged dysregulation of signalling and β-cell metabolic control leads to β-cell dysfunction, and is increasingly associated with abnormal metabolic states which disrupt normal cellular physiology. Utilization of appropriate β-cell models enables a systematic approach to understand the impact of perturbations to the biological system. The BRIN-BD11 β-cell line is a useful, pre-clinical cell model for β-cell dysfunction associated with type 2 diabetes, among other metabolic disorders. The present chapter describes detection and analysis of β-cell dysfunction with respect to changes in bioenergetics and metabolism, generation of intracellular reactive oxygen species, and acute and chronic insulin secretion in the BRIN-BD11 cell line.",
keywords = "BRIN-BD11 β-cells, Bioenergetics, Diabetes, Insulin, Metabolism",
author = "Jordan Rowlands and Nikita Walz and Rowles, {Joanne E.} and Keane, {Kevin N.} and Rodrigo Carlessi and Philip Newsholme",
note = "Publisher Copyright: {\textcopyright} 2019, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2019",
doi = "10.1007/978-1-4939-8994-2_32",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "329--340",
booktitle = "Methods in Molecular Biology",
}