Abstract
Separation of model membrane vesicles from external solution is an important step in quantitation of vesicle-associated materials. Ordinarily, such experiments cannot be done with small unilamellar vesicles because they are too small to be pelleted by centrifugation. A method is described that overcomes this difficulty by use of brominated phospholipids. It is shown that efficient pelleting of small unilamellar vesicles (greater than 98%) can be obtained by ultracentrifugation.
| Original language | English |
|---|---|
| Pages (from-to) | 176-180 |
| Number of pages | 5 |
| Journal | Analytical Biochemistry |
| Volume | 217 |
| Issue number | 2 |
| DOIs | |
| State | Published - Mar 1994 |
| Externally published | Yes |