Method for efficient pelleting of small unilamellar model membrane vesicles

Domenico Tortorella, Erwin London

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Separation of model membrane vesicles from external solution is an important step in quantitation of vesicle-associated materials. Ordinarily, such experiments cannot be done with small unilamellar vesicles because they are too small to be pelleted by centrifugation. A method is described that overcomes this difficulty by use of brominated phospholipids. It is shown that efficient pelleting of small unilamellar vesicles (greater than 98%) can be obtained by ultracentrifugation.

Original languageEnglish
Pages (from-to)176-180
Number of pages5
JournalAnalytical Biochemistry
Volume217
Issue number2
DOIs
StatePublished - Mar 1994
Externally publishedYes

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