TY - JOUR
T1 - Membrane protein structure determination by electron crystallography
AU - Ubarretxena-Belandia, Iban
AU - Stokes, David L.
N1 - Funding Information:
The authors thank Dr. Andreas Engel for providing the diffraction pattern and molecular structure used in Box 1 . The authors gratefully acknowledge support from the National Institutes of Health ( R01 GM095747 and U54 GM094598 ).
PY - 2012/8
Y1 - 2012/8
N2 - During the past year, electron crystallography of membrane proteins has provided structural insights into the mechanism of several different transporters and into their interactions with lipid molecules within the bilayer. From a technical perspective there have been important advances in high-throughput screening of crystallization trials and in automated imaging of membrane crystals with the electron microscope. There have also been key developments in software, and in molecular replacement and phase extension methods designed to facilitate the process of structure determination.
AB - During the past year, electron crystallography of membrane proteins has provided structural insights into the mechanism of several different transporters and into their interactions with lipid molecules within the bilayer. From a technical perspective there have been important advances in high-throughput screening of crystallization trials and in automated imaging of membrane crystals with the electron microscope. There have also been key developments in software, and in molecular replacement and phase extension methods designed to facilitate the process of structure determination.
UR - http://www.scopus.com/inward/record.url?scp=84865287659&partnerID=8YFLogxK
U2 - 10.1016/j.sbi.2012.04.003
DO - 10.1016/j.sbi.2012.04.003
M3 - Review article
C2 - 22572457
AN - SCOPUS:84865287659
SN - 0959-440X
VL - 22
SP - 520
EP - 528
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
IS - 4
ER -