Mechanical loading differentially regulates membrane-bound and soluble RANKL availability in MC3T3-E1 cells

Dae Won Kim; Hahn Jun Lee; Jaime A. Karmin; Se Eun Lee; Seong Sil Chang; Ben Tolchin; Shiphin Lin; Sam K. Cho; Anthony Kwon; Jae Mok Ahn; Francis Young In Lee

doi:10.1196/annals.1346.054

Mechanical loading differentially regulates membrane-bound and soluble RANKL availability in MC3T3-E1 cells

Dae Won Kim, Hahn Jun Lee, Jaime A. Karmin, Se Eun Lee, Seong Sil Chang, Ben Tolchin, Shiphin Lin, Sam K. Cho, Anthony Kwon, Jae Mok Ahn, Francis Young In Lee

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

To understand the biochemical response of RANKL in response to mechanical loading, MC3T3-E1 cells were biequiaxially stretched. A murine RANKL cDNA with double epitopes, pEF6 HA-RANKL-V5His, was transfected into MC3T3-E1 cells, which were then stretched. Endogenous RANKL protein expression increased in response to mechanical loading. Membrane-bound RANKL (HA-RANKL-V5His) increased in cell lysates while soluble RANKL (RANKL-V5His) decreased in the conditioned media after mechanical loading. This may have resulted from the decreased activity of TACE after mechanical loading. Increased membrane-bound RANKL may be one of the mechanisms through which osteoblasts adapt to mechanical loading by regulating osteoclastogenic activity in a region-specific manner.

Original language	English
Pages (from-to)	568-572
Number of pages	5
Journal	Annals of the New York Academy of Sciences
Volume	1068
Issue number	1
DOIs	https://doi.org/10.1196/annals.1346.054
State	Published - Apr 2006
Externally published	Yes

Keywords

Mechanical loading
Osteoblast
RANKL
TACE

Access to Document

10.1196/annals.1346.054

Cite this

@article{24bbe8bd2b9342ac929a1854d7ea3cae,

title = "Mechanical loading differentially regulates membrane-bound and soluble RANKL availability in MC3T3-E1 cells",

abstract = "To understand the biochemical response of RANKL in response to mechanical loading, MC3T3-E1 cells were biequiaxially stretched. A murine RANKL cDNA with double epitopes, pEF6 HA-RANKL-V5His, was transfected into MC3T3-E1 cells, which were then stretched. Endogenous RANKL protein expression increased in response to mechanical loading. Membrane-bound RANKL (HA-RANKL-V5His) increased in cell lysates while soluble RANKL (RANKL-V5His) decreased in the conditioned media after mechanical loading. This may have resulted from the decreased activity of TACE after mechanical loading. Increased membrane-bound RANKL may be one of the mechanisms through which osteoblasts adapt to mechanical loading by regulating osteoclastogenic activity in a region-specific manner.",

keywords = "Mechanical loading, Osteoblast, RANKL, TACE",

author = "Kim, {Dae Won} and Lee, {Hahn Jun} and Karmin, {Jaime A.} and Lee, {Se Eun} and Chang, {Seong Sil} and Ben Tolchin and Shiphin Lin and Cho, {Sam K.} and Anthony Kwon and Ahn, {Jae Mok} and Lee, {Francis Young In}",

year = "2006",

month = apr,

doi = "10.1196/annals.1346.054",

language = "English",

volume = "1068",

pages = "568--572",

journal = "Annals of the New York Academy of Sciences",

issn = "0077-8923",

publisher = "New York Academy of Sciences",

number = "1",

}

TY - JOUR

T1 - Mechanical loading differentially regulates membrane-bound and soluble RANKL availability in MC3T3-E1 cells

AU - Kim, Dae Won

AU - Lee, Hahn Jun

AU - Karmin, Jaime A.

AU - Lee, Se Eun

AU - Chang, Seong Sil

AU - Tolchin, Ben

AU - Lin, Shiphin

AU - Cho, Sam K.

AU - Kwon, Anthony

AU - Ahn, Jae Mok

AU - Lee, Francis Young In

PY - 2006/4

Y1 - 2006/4

N2 - To understand the biochemical response of RANKL in response to mechanical loading, MC3T3-E1 cells were biequiaxially stretched. A murine RANKL cDNA with double epitopes, pEF6 HA-RANKL-V5His, was transfected into MC3T3-E1 cells, which were then stretched. Endogenous RANKL protein expression increased in response to mechanical loading. Membrane-bound RANKL (HA-RANKL-V5His) increased in cell lysates while soluble RANKL (RANKL-V5His) decreased in the conditioned media after mechanical loading. This may have resulted from the decreased activity of TACE after mechanical loading. Increased membrane-bound RANKL may be one of the mechanisms through which osteoblasts adapt to mechanical loading by regulating osteoclastogenic activity in a region-specific manner.

AB - To understand the biochemical response of RANKL in response to mechanical loading, MC3T3-E1 cells were biequiaxially stretched. A murine RANKL cDNA with double epitopes, pEF6 HA-RANKL-V5His, was transfected into MC3T3-E1 cells, which were then stretched. Endogenous RANKL protein expression increased in response to mechanical loading. Membrane-bound RANKL (HA-RANKL-V5His) increased in cell lysates while soluble RANKL (RANKL-V5His) decreased in the conditioned media after mechanical loading. This may have resulted from the decreased activity of TACE after mechanical loading. Increased membrane-bound RANKL may be one of the mechanisms through which osteoblasts adapt to mechanical loading by regulating osteoclastogenic activity in a region-specific manner.

KW - Mechanical loading

KW - Osteoblast

KW - RANKL

KW - TACE

UR - http://www.scopus.com/inward/record.url?scp=33744749571&partnerID=8YFLogxK

U2 - 10.1196/annals.1346.054

DO - 10.1196/annals.1346.054

M3 - Article

C2 - 16831954

AN - SCOPUS:33744749571

SN - 0077-8923

VL - 1068

SP - 568

EP - 572

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

IS - 1

ER -

Mechanical loading differentially regulates membrane-bound and soluble RANKL availability in MC3T3-E1 cells

Abstract

Keywords

Access to Document

Fingerprint

Cite this