Measuring uptake dynamics of multiple identifiable carbon nanotube species via high-speed confocal Raman imaging of live cells

Jeon Woong Kang, Freddy T. Nguyen, Niyom Lue, Ramachandra R. Dasari, Daniel A. Heller

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Carbon nanotube uptake was measured via high-speed confocal Raman imaging in live cells. Spatial and temporal tracking of two cell-intrinsic and nine nanotube-derived Raman bands was conducted simultaneously in RAW 264.7 macrophages. Movies resolved single (n, m) species, defects, and aggregation states of nanotubes transiently as well as the cell position, denoted by lipid and protein signals. This work portends the real-time molecular imaging of live cells and tissues using Raman spectroscopy, affording multiplexing and complete photostability.

Original languageEnglish
Pages (from-to)6170-6174
Number of pages5
JournalNano Letters
Volume12
Issue number12
DOIs
StatePublished - 12 Dec 2012
Externally publishedYes

Keywords

  • Raman microscopy
  • Raman spectroscopy
  • Single-walled carbon nanotube
  • intracellular trafficking
  • molecular probe
  • resonance Raman scattering

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