Abstract
Carbon nanotube uptake was measured via high-speed confocal Raman imaging in live cells. Spatial and temporal tracking of two cell-intrinsic and nine nanotube-derived Raman bands was conducted simultaneously in RAW 264.7 macrophages. Movies resolved single (n, m) species, defects, and aggregation states of nanotubes transiently as well as the cell position, denoted by lipid and protein signals. This work portends the real-time molecular imaging of live cells and tissues using Raman spectroscopy, affording multiplexing and complete photostability.
Original language | English |
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Pages (from-to) | 6170-6174 |
Number of pages | 5 |
Journal | Nano Letters |
Volume | 12 |
Issue number | 12 |
DOIs | |
State | Published - 12 Dec 2012 |
Externally published | Yes |
Keywords
- Raman microscopy
- Raman spectroscopy
- Single-walled carbon nanotube
- intracellular trafficking
- molecular probe
- resonance Raman scattering