Measurement of NitriteNitrite in Blood Samples Using the Ferricyanide-Based Hemoglobin OxidationOxidation Assay

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

39 Scopus citations

Abstract

NitriteNitrite is currently recognized as a biomarker of the state of nitric oxide metabolism. Therefore, assessing nitrite levels in various organs and compartments is an important issue. As nitrite levels in most organs and tissues are low (in high nanomolar or low micromolar range) several new sensitive methods for quantifying nitrite in various biological samples have been developed. ChemiluminescenceChemiluminescence, combined with tri-iodide reducing solution, is currently considered the most sensitive method, allowing quantification in the low nanomolar range of nitrite concentrations. Here, we present an overview of chemiluminescence-based determination of nitrite in blood and blood compartments – red blood cellsred blood cells and plasma. We also explain how to preserve the original physiological nitrite concentration in nitrite-hostile environments, such as an excess of hemoglobin in blood.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages39-56
Number of pages18
DOIs
StatePublished - 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume704
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Nitrite
  • chemiluminescence
  • hemoglobin
  • red blood cell

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